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Effect of ascorbic acid administration on erythrocyte osmotic fragility of pigs transported by road during the hot-dry season

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Abstract

The experiments were performed in order to determine the effect of 8-h road transportation of pigs on erythrocytes osmotic fragility during the hot-dry season, and the ameliorative role of ascorbic acid. Twenty-three adult pigs comprising of both sexes served as subjects for the study. Thirteen pigs administered ascorbic acid (AA) per os 30 min before transportation, at a dose rate of 100 mg/kg served as experimental animals, while ten pigs administered with distilled water per os served as control, and were transported for 8 h during the hot-dry season. EDTA blood samples collected a day before (pre-transportation), immediately after 8-h transportation and 7 days post-transportation were used to determine erythrocyte osmotic fragility. The ambient temperature (AT) and relative humidity (RH) measured within the vehicle ranged between 30.5 – 39.0°C and 40.0 – 71.0% respectively. These values were outside the thermoneutral zone for the pig, indicating that the season was thermally stressful. Results obtained showed a significant difference (p < 0.05) in percent haemolysis recorded at NaCl concentrations of 0.4% and 0.6% immediately after transportation in experimental pigs and at 0.5, 0.6, 0.8 and 0.9% NaCl concentrations in experimental pigs 7 days post-transportation. In conclusion, result from the present study indicated that 8-h road transportation during the hot-dry season could induce stress resulting in haemolysis of erythrocytes and AA administration ameliorated the stress.

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Authors and Affiliations

  1. National Veterinary Research Institute, P. M. B. 01, Vom, Plateau State, Nigeria

    Olayinka O. Asala

  2. Department of Veterinary Physiology and Pharmacology, Ahmadu Bello University, Zaria, Nigeria

    Joseph O. Ayo

  3. National Animal Production Research Institute, Shika, Zaria, Nigeria

    Peter I. Rekwot

  4. College of Agriculture and Animal Science, Ahmadu Bello University, Kaduna, Nigeria

    Ndazo S. Minka & David O. Omoniwa

  5. Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Agriculture, Makurdi, Nigeria

    Adeshina Y. Adenkola

Authors
  1. Olayinka O. Asala
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  2. Joseph O. Ayo
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  3. Peter I. Rekwot
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  4. Ndazo S. Minka
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  5. David O. Omoniwa
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  6. Adeshina Y. Adenkola
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Correspondence to Olayinka O. Asala.

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Asala, O.O., Ayo, J.O., Rekwot, P.I. et al. Effect of ascorbic acid administration on erythrocyte osmotic fragility of pigs transported by road during the hot-dry season. Vet Res Commun 35, 245–254 (2011). https://doi.org/10.1007/s11259-011-9469-7

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  • DOI: https://doi.org/10.1007/s11259-011-9469-7

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