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Proteomic analysis of mammary tissues from healthy cows and clinical mastitic cows for identification of disease-related proteins

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Abstract

To investigate the disease-related proteins and understand molecular mechanism of mastitis at the protein level, this project presents the protein changes in the mammary gland between healthy cows and clinical mastitic cows using two-dimensional gel electrophoresis (2-DE), after stained with colloidal Coomassie Bright Blue, six spots of differentially expressed protein were detected by PDQuest software and subjected to ion trap mass spectrometer equipped with a HPLC system, and five proteins were identified. Hemoglobin beta, kappa-casein and tryptophanyl-tRNA-synthetase (TrpRS) in healthy dairy cows, while hemoglobin beta, cytochrome C oxidase and annexin V in clinical mastitic cows were identified, they were involved in binding, transport and catalytic activity. The results may provide valuable information for the investigating of the host mammary immune system response to defense against pathogens at the protein level and potential protein targets for treatment.

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Abbreviations

2-DE:

two-dimensional gel electrophoresis

IEF:

isoelectric focusing

LMT:

Lanzhou Mastitis Test

MS/MS:

tandem mass spectrometery

Mw:

molecular weight

m/z:

mass/charge

pI:

isoelectric point

TrpRS:

tryptophanyl-tRNA-synthetase

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Acknowledgements

The project was supported by the China International S&T Cooperation project (2005 DFA30720). We thank Dr. Xiaoming Li, Beijing Institute of Zoology, Chinese Academy of Sciences, for his technical assistance in ion trap spectrometer.

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Authors and Affiliations

  1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, China

    Yong-xin Yang, Xing-xu Zhao & Yong Zhang

  2. Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural sciences, Hefei, 230031, China

    Yong-xin Yang

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  1. Yong-xin Yang
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  2. Xing-xu Zhao
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Correspondence to Xing-xu Zhao.

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Yang, Yx., Zhao, Xx. & Zhang, Y. Proteomic analysis of mammary tissues from healthy cows and clinical mastitic cows for identification of disease-related proteins. Vet Res Commun 33, 295–303 (2009). https://doi.org/10.1007/s11259-008-9177-0

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  • DOI: https://doi.org/10.1007/s11259-008-9177-0

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