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Equine adipose-tissue derived mesenchymal stem cells and platelet concentrates: their association in vitro and in vivo

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Abstract

Equine mesenchymal stem cells (MSC) are of particular interest both for basic research and for the therapeutic approach to musculoskeletal diseases in the horse. Their multilineage differentiation potential gives them the capability to contribute to the repair of tendon, ligament and bone damage. MSCs are also considered a promising therapeutic aid in allogeneic cell transplantation, since they show low immunogenicity and immunomodulating functions.

Adipose tissue-derived adult equine stem cells (AdMSC) can be isolated, expanded in vitro and then inoculated into the damaged tissue, eventually in the presence of a biological scaffold. Here we report our preliminary experience with adipose-derived mesenchymal stem cells in allogeneic cell-therapy of tendonitis in the horse. MSCs, derived from visceral adipose tissue, were grown in the presence of autologous platelet lysate and characterized for their differentiation and growth potential. Expanded AdMSC were inoculated into the damaged tendon after their dispersion in activated platelet-rich plasma (PRP), a biological scaffold that plays an important role in maintaining cells in defect sites and contributes to tissue healing. Fourteen out of sixteen treated horses showed a functional recovery and were able to return to their normal activity.

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References

  • Chong A.K., Ang A.D., Goh J.C., Hui J.H., Lim A.Y., Lee E.H., Lim B.H., 2007. Bone marrow-derived mesenchymal stem cells influence early tendon-healing in a rabbit achilles tendon model. Journal of Bone and Joint Surgery. American volume, 89(1), 74–81.

    Article  Google Scholar 

  • Crovace A., Lacitignola L., De Siena R., Rossi G., Francioso E., 2007. Cell therapy for tendon repair in horses: an experimental study. Veterinary Research Communications, Suppl 1, 281–283.

    Article  Google Scholar 

  • Datta S.C. and Naryanaswami A., 1983. Fluorometric estimation of taurine in tissue extracts and biological fluids. Italian Journal of Biochemistry, 32(5), 297–305.

    PubMed  CAS  Google Scholar 

  • Del Bue M., Riccò S., Conti V., Merli E., Ramoni R., Grolli S., 2007. Platelet lysate promotes in vitro proliferation of equine mesenchymal stem cells and tenocytes. Veterinary Research Communications, Suppl 1, 289–292.

    Google Scholar 

  • Gillis C., 2005, Soft tissue injuries: tendonitis and desmitis. In Equine Sports Medicine and Surgery, Hinchcliff K (Ed.), Sounders Comp.: Philadelphia, 412–432.

  • Luengo Gimeno F., Gatto S., Ferro J., Croxatto J.O., Gallo J.E., 2006. Preparation of platelet-rich plasma as a tissue adhesive for experimental transplantation in rabbits. Thrombosis Journal, 28, 4–18.

    Google Scholar 

  • Molloy T., Wang Y., Murrell G., 2003. The roles of growth factors in tendon and ligament healing. Sports Medicine, 33(5), 381–394.

    Article  PubMed  Google Scholar 

  • Natua A.J. and Fibbe W.E., 2007. Immunomodulatory properties of mesenchymal stromal cells. Blood, 110(10), 3499–3506.

    Article  Google Scholar 

  • Nixon A.J., Goodrich L.R., Scimeca M.S., Witte T.H., Schnabel L.V., Watts A.E., Robbins P.D., 2007. Gene therapy in musculoskeletal repair. Annals of the New York Academy of Sciences, 1117, 310–327.

    Article  PubMed  CAS  Google Scholar 

  • Richardson L.E., Dudhia J., Clegg P.D., Smith R., 2007. Stem cells in veterinary medicine: attempts at regenerating equine tendon after injury. Trends in Biotechnology, 25(9), 409–416.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Animal Health, Veterinary Biotechnology, Food Quality and Safety, University of Parma, via del Taglio 8, 43100, Parma, Italy

    M. Del Bue, S. Riccò & G. Gnudi

  2. Department of Animal Production, Veterinary Biotechnology, Food Quality and Safety, University of Parma, via del Taglio 8, 43100, Parma, Italy

    R. Ramoni, V. Conti & S. Grolli

Authors
  1. M. Del Bue
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  2. S. Riccò
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  3. R. Ramoni
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  4. V. Conti
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  5. G. Gnudi
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  6. S. Grolli
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Corresponding author

Correspondence to S. Grolli.

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Del Bue, M., Riccò, S., Ramoni, R. et al. Equine adipose-tissue derived mesenchymal stem cells and platelet concentrates: their association in vitro and in vivo . Vet Res Commun 32 (Suppl 1), 51–55 (2008). https://doi.org/10.1007/s11259-008-9093-3

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  • DOI: https://doi.org/10.1007/s11259-008-9093-3

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