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Stimulation of the In Vitro Migration of Ovine Eosinophils by Factors Derived from the Sheep Scab Mite, Psoroptes ovis

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Abstract

The ectoparasitic astigmatid mite Psoroptes ovis causes sheep scab, a highly contagious, severe allergic dermatitis associated with damage to the fleece and hide, loss of condition and occasional mortality. The scab lesion is characterized by a massive infiltration of eosinophils that begins very rapidly after infection. This paper reports the finding that mite-derived factors directly enhance the migration of ovine eosinophils in vitro. Significant (p < 0.01) and dose-dependent (r = 0.972 ± 0.018 (SD)) activity was initially identified in whole mite extracts, by comparison with medium controls in an assay based on modified Boyden chambers and ovine bone marrow target cells. Similar pro-migratory activity (p < 0.005; r = 0.928 ± 0.069 (SD)) was detected in washes containing mite excretory/secretory material. By direct comparison with migration ratios (n = 3) for defined chemotactic (rmeotaxin = 3.430 ± 0.360 (SD)) and chemokinetic (rminterleukin-5 = 0.982 ± 0.112 (SD)) stimuli it was determined that the activity in both mite extracts (0.992 ± 0.038 (SD)) and mite washes (0.969 ± 0.071 (SD)) was chemokinetic. Subsequent experiments (n = 3) in which live mites were incorporated directly into the in vitro assay system indicated that they produced factors that significantly (p < 0.001) enhanced eosinophil migration to a degree directly related to mite numbers (r = 0.993 ± 0.005 (SD)). The identity of the factor(s) responsible is uncertain, but their presence suggests that mites may be capable of directly activating eosinophils in vivo, and raises the possibility that mites could directly influence, perhaps even initiate, the rapid early tissue eosinophilic response observed in experimental sheep scab infections.

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Abbreviations

FCS:

fetal calf serum

HBSS:

Hanks balanced salt solution

IL-5:

interleukin-5

IMDM:

Iscove’s modified Dulbecco’s medium

PBS:

phosphate-buffered saline

r :

correlation coefficient

rm:

recombinant murine

SBMC:

sheep bone marrow cells

SD:

standard deviation

SDS:

sodium dodecyl sulphate

SEM:

standard error of the mean

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  1. Moredun Research Institute, Pentlands Science Park, Penicuik, Scotland, UK

    L. A. Wildblood & D. G. Jones

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  1. L. A. Wildblood
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  2. D. G. Jones
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Correspondence to D. G. Jones.

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Wildblood, L.A., Jones, D.G. Stimulation of the In Vitro Migration of Ovine Eosinophils by Factors Derived from the Sheep Scab Mite, Psoroptes ovis . Vet Res Commun 31, 197–206 (2007). https://doi.org/10.1007/s11259-006-3429-7

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  • DOI: https://doi.org/10.1007/s11259-006-3429-7

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