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Immune Responses and Protective Efficacy of Binary Ethylenimine (BEI)-Inactivated Bluetongue Virus Vaccines in Sheep

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Abbreviations

Al(OH)3 :

aluminium hydroxide

BEI:

binary ethylenimine

BTV:

bluetongue virus

CMI:

cell-mediated immune response

CPE:

cytopathic effect

CRI:

clinical reaction index

d.p.i.:

days post inoculation

DTH:

delayed-type hypersensitivity

EHDV:

epizootic haemorrhagic disease virus of deer

FIA:

Freund’s incomplete adjuvant

PBS:

phosphate-buffered saline

pfu:

plaque-forming unit

PHA:

phytohaemagglutinin

RBC:

red blood cell

References

  • Bahnemann, H.G., 1990. Inactivation of viral antigens for vaccine preparation with particular reference to the application of binary ethylenimine. Vaccine, 8, 299–303

    Article  PubMed  CAS  Google Scholar 

  • Huismans, H., Van Der Walt, N.T., Cloete, M. and Erasmus, B.J., 1987. Isolation of capsid protein of bluetongue virus that induces a protective immune response in sheep. Virology, 157, 172–179

    Article  PubMed  CAS  Google Scholar 

  • Jeggo, M.H., Wardley, R.C. and Brownlie, J., 1984. A study of the role of cell-mediated immunity in bluetongue infection in sheep, using cellular adoptive transfer techniques. Immunology, 52, 403–410

    PubMed  CAS  Google Scholar 

  • Johnson, S.J. and Roy, P., 1996. Bluetongue recombinant vaccines. In: T.D. St.George and P. Kegao (eds), Proceedings of the First Southeast Asia and Pacific Regional Bluetongue Symposium, Kunming, P.R. China, 1995, (ACIAR Proceedings no. 66), 192–197

  • Mahrt, C.R. and Osburn, B.I., 1986. Experimental bluetongue virus infection in sheep; effect of previous vaccination: clinical and immunologic studies. American Journal of Veterinary Research, 47, 1191–1197

    PubMed  CAS  Google Scholar 

  • Murray, P.K. and Eaton, B.T., 1996. Vaccines for bluetongue. Australian Veterinary Journal, 73, 207–210

    PubMed  CAS  Google Scholar 

  • Odeon, A.C., Gershwin, L.J. and Osburn, B.I., 1999. IgE responses to bluetongue virus (BTV) serotype 11 after immunization with inactivated BTV and challenge infection. Comparative Immunology, Microbiology and Infectious Diseases, 22, 145–162

    Article  PubMed  CAS  Google Scholar 

  • Pierce C.M., Balasuriya, U.B.R. and MacLachlan, M.J., 1998. Phylogenetic analysis of the S 10 gene of field and laboratory strains of bluetongue virus from the United States. Virus Research, 55, 15–27

    Article  PubMed  CAS  Google Scholar 

  • Quist, C.F., Howerth, E.W., Bounous, D.I. and Stallknecht, D.E., 1997. Cell-mediated immune response and IL-2 production in white-tailed deer experimentally infected with haemorrhagic disease viruses. Veterinary Immunology and Immunopathology, 56, 283–297

    Article  PubMed  CAS  Google Scholar 

  • Ramakrishnan, M.A., Singh, K.P., Pandey, A.B., Singh, R., Nandi, S. and Mehrotra, M.L., 2003. Analysis of RNA of inactivated bluetongue virus vaccines by RNA-polyacrylamide gel electrophoresis (PAGE). In: 8th International Symposium on Double-Stranded RNA Viruses, 2003, II Ciocco, Castelvecchio Pascoli, Italy, 125

    Google Scholar 

  • Richards, R.G., MacLachlan, N.J., Heidner, H.W. and Fuller, F.J., 1988. Comparison of virologic and serologic responses of lambs and calves infected with bluetongue virus serotype 10. Veterinary Microbiology, 18, 233–242

    Article  PubMed  CAS  Google Scholar 

  • Roy, P., 1996. Development of bluetongue virus multicomponent vaccines using a novel baculovirus system. In: T.D. St.George and P. Kegao (eds), Proceedings of the First Southeast Asia and Pacific Regional Bluetongue Symposium, Kunming, P.R. China, 1995, (ACIAR Proceedings no. 66), 183–191

  • Sreenivasalu, D., Rao, M.V.S. and Gard, G.P., 1996. Bluetongue viruses in India: a review. In: T.D. St.George and P. Kegao (eds), Proceedings of the First Southeast Asia and Pacific Regional Bluetongue Symposium, Kunming, P.R. China, 1995, (ACIAR Proceedings no. 66), 15–20

  • Stott, J.L., Barber, T.L. and Osburn, B.I., 1985. Immunologic response of sheep to inactivated and virulent bluetongue virus. American Journal of Veterinary Research, 45, 1043–1049

    Google Scholar 

  • Uppal, P.K., 1992. Present position of African horse sickness and Bluetongue in India. In: T.E. Walton and B.I. Osburn (eds), Bluetongue, African Horse Sickness and Related Orbiviruses, (Boca Raton, FL: CRC Press), 175–190

    Google Scholar 

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Authors and Affiliations

  1. Centre for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar, UP, 243122, India

    M. A. Ramakrishnan, A. B. Pandey, K. P. Singh, R. Singh, S. Nandi & M. L. Mehrotra

Authors
  1. M. A. Ramakrishnan
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  2. A. B. Pandey
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  3. K. P. Singh
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  4. R. Singh
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  5. S. Nandi
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  6. M. L. Mehrotra
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Corresponding author

Correspondence to M. A. Ramakrishnan.

Additional information

Ramakrishnan, M.A., Pandey, A.B., Singh, K.P., Singh, R., Nandi, S. and Mehrotra, M.L., 2006. Immune responses and protective efficacy of binary ethylenimine (BEI)-inactivated bluetongue virus vaccines in sheep. Veterinary Research Communications, 30(8), 873–880

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Ramakrishnan, M.A., Pandey, A.B., Singh, K.P. et al. Immune Responses and Protective Efficacy of Binary Ethylenimine (BEI)-Inactivated Bluetongue Virus Vaccines in Sheep. Vet Res Commun 30, 873–880 (2006). https://doi.org/10.1007/s11259-006-3313-5

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  • DOI: https://doi.org/10.1007/s11259-006-3313-5

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