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Cultural and Immunological Detection Methods for Salmonella spp. in Animal Feeds – A Review

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Abstract

Food-borne salmonellosis continues to be a major public health concern, and contamination with Salmonella spp. in pre-harvest animal production is considered a primary contributor to this problem. Animal feeds can easily become contaminated during primary production, feed mixing and processing as well as during feeding. Consequently, monitoring and surveillance of feeds and feed ingredients for Salmonella spp. contamination may be useful or necessary in the prevention and control of this organism. Cultural and immunological detection methods for salmonellae have been used or suggested as possible approaches for use in animal feeds. Cultural methods remain advantageous owing to their ability to detect viable bacterial cells, while immunological methods have the capability of detecting nonculturable bacterial cells. Advancements and improvements in both methodologies offer opportunities for eventual routine use of these detection technologies in animal feed assays.

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Abbreviations

cfu:

colony-forming unit(s)

ELISA:

enzyme-linked immunosorbent assay

FDA:

US Food and Drug Administration

FSIS:

USDA Food Safety and Inspection Service

BG:

Brilliant green

HE:

Hektoen enteric

IgM:

immunoglobulin M

IMS:

immunomagnetic separation

MSRV:

modified semisolid RV

S.:

Salmonella

SC:

selenite cystine

RV:

Rappaport–Vassiliadis

TSA:

tryptic soy agar

TT:

tetrathionate

USDA:

US Department of Agriculture

XLD:

xylose lysine desoxycholate

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Author notes

  1. K. G. Maciorowski

    Present address: Department of Agriculture and Natural Resources, Delaware State University, Dover, Delaware, USA

Authors and Affiliations

  1. Poultry Science Department, Texas A&M University, College Station, TX, 77843-2472, USA

    K. G. Maciorowski, P. Herrera, S. D. Pillai & S. C. Ricke

  2. Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, Arkansas, USA

    F. T. Jones

Authors
  1. K. G. Maciorowski
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  2. P. Herrera
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  3. F. T. Jones
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  4. S. D. Pillai
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  5. S. C. Ricke
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Correspondence to S. C. Ricke.

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Maciorowski, K.G., Herrera, P., Jones, F.T. et al. Cultural and Immunological Detection Methods for Salmonella spp. in Animal Feeds – A Review. Vet Res Commun 30, 127–137 (2006). https://doi.org/10.1007/s11259-006-3221-8

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