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Serological Investigation and Genomic Characterization of PCV2 Isolates from Different Geographic Regions of Zhejiang Province in China

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Abstract

Sera collected from 46 swine farms in Zhejiang province were evaluated for the presence of antibodies to PCV2 using an indirect-fluorescent antibody procedure. In addition PCV2 isolated from superficial inguinal lymph node samples collected from 40-to 90-day-old pigs with clinical signs of post-weaning multisystemic wasting syndrome (PMWS) using the PK-15 cell line were sequenced and compared. Overall seroprevalence of PCV2 antibody averaged 58.34% for all samples. Breakdown of serology by groups was as follows: 59.38% for sows, 57.41% for post-weaning piglets, 44.83% for Landrace sows and 64.28% for Landrace piglets. The seroprevalence of Landrace sows was higher than that of Yorkshire and Duroc sows, but non-significant (p > 0.05). Serological analysis also showed that seroprevalence of PCV2 antibody was a negative correlation to that of PRRSV antibody. The complete genomes of five PCV2 isolates identified in the herds with PMWS consisted of 1767nt, containing the 11 potential ORFs. Genome of the virus isolates shared 93.8% to 99.8% identity with PCV2 reference strains from GenBank, 76.6% to 77.9% identity with PCV1. Phylogenetic analysis indicated that there were two subgenotypes within PCV2: subgenotype I (1767 nt) and subgenotype II (1768 nt).

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Abbreviations

aa:

amino acid residue

EDTA:

ethylenediaminetetraacetic acid

ELISA:

enzyme-linked immunosorbent assay

FITC:

fluorescein isothicyanate

FBS:

fetalbovine serum

IFA:

indirect immunofluoresent assay

ISU VDL:

Veterinary Diagnostic Laboratory, Iowa State University

MEM:

minimum essential medium

nt:

nucleotides

ORF:

open reading frame

PBS:

phosphate-buffiered saline

PCR:

polymerase chain reaction

PCV:

porcine circovirus

PCV1:

porcine circovirus type 1

PCV2:

porcine circovirus type 2

PK-15:

porcine kidney 15 cell line

PWMS:

post-weaning multisystemic wasting syndrome

PRRSV:

porcine reproductive and respiratory syndrome virus

PrV:

pseudorabies virus

TCID50 :

median tissue culture infectious dose

RNA:

ribonucleic acid

IU:

international unit

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Authors and Affiliations

  1. Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310029, Peoples Republic of China

    J.-Y. Zhou, Q.-X. Chen, J.-X. Ye, H.-G. Shen, T.-F. Chen & S.-B. Shang

  2. State Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Hangzhou, 310029, Peoples Republic of China

    J.-Y. Zhou

  3. Key Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals of the Ministry of Education, Zhejiang University, Hangzhou, 310029, Peoples Republic of China

    J.-Y. Zhou

  4. College of Life Sciences, Zhejiang University, Hangzhou, 310029, Peoples Republic of China

    Q.-X. Chen

  5. Institute of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang University, 268 Kaixuan Road, Hangzhou, Zhejiang, 310029, Peoples Republic of China

    J.-Y. Zhou

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  1. J.-Y. Zhou
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  2. Q.-X. Chen
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Correspondence to J.-Y. Zhou.

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Zhou, JY., Chen, QX., Ye, JX. et al. Serological Investigation and Genomic Characterization of PCV2 Isolates from Different Geographic Regions of Zhejiang Province in China. Vet Res Commun 30, 205–220 (2006). https://doi.org/10.1007/s11259-006-3203-x

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  • DOI: https://doi.org/10.1007/s11259-006-3203-x

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