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Inhibiting malignant phenotypes of the bladder cancer cells by silencing long noncoding RNA SChLAP1

  • Urology - Original Paper
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Abstract

Background and objectives

Long noncoding RNAs (lncRNAs) play key roles in process of cancer cell growth and apoptosis and have received increasing attention. SChLAP1 is a novel lncRNA that is required for development and progression of prostate cancer. We hypothesized that SChLAP1 also has important biological functions in human bladder cancer which is another type of urological cancer.

Methods

The expression of SChLAP1 in bladder cancer was determined using real-time qPCR. Bladder cancer T24 and 5637 cells were transfected with SChLAP1 siRNA or negative control siRNA. Cell proliferation, apoptosis and migration were determined using CCK-8 assay, flow cytometry analysis and wound healing assay, respectively.

Results

SChLAP1 was overexpressed in bladder cancer tissues compared to paired normal bladder tissues. Cell growth arrest, apoptosis induction and migration inhibition were also observed in bladder cancer T24 and 5637 cells after transfection with SChLAP1 siRNA.

Conclusions

Our data suggest that SChLAP1 plays oncogenic roles and can be used as a therapeutic target for treating human bladder cancer.

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    Authors and Affiliations

    1. Department of Urology Surgery Center, The People’s Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, 830002, Xinjiang, People’s Republic of China

      Jianjun Zhang, Zhenfeng Shi, Yukui Nan & Ming Li

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    1. Jianjun Zhang
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    2. Zhenfeng Shi
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    3. Yukui Nan
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    4. Ming Li
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    Corresponding author

    Correspondence to Ming Li.

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    All authors declare that they have no conflict of interest.

    Ethical approval

    All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

    Informed consent

    Informed consent was obtained from all individual participants included in the study.

    Additional information

    Jianjun Zhang and Zhenfeng Shi have contributed equally to this work.

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    Cite this article

    Zhang, J., Shi, Z., Nan, Y. et al. Inhibiting malignant phenotypes of the bladder cancer cells by silencing long noncoding RNA SChLAP1. Int Urol Nephrol 48, 711–716 (2016). https://doi.org/10.1007/s11255-016-1230-2

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    • DOI: https://doi.org/10.1007/s11255-016-1230-2

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