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Will metformin postpone high-fat diet promotion of TRAMP mouse prostate cancer development and progression?

  • Urology - Original Paper
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Abstract

Purpose

We aimed to examine the effect of high-fat diet (HFD) on prostate cancer (PCa) development and progression and to investigate whether metformin would postpone PCa development and progression promoted by HFD.

Methods

TRAMP mice were randomly divided into three groups: normal diet group, HFD group and metformin-HFD (Met-HFD) group. Mortality rate and tumor formation rate were examined. TRAMP mice were sacrificed and sampled on the 20th, 24th, and 28th week, respectively. Serum levels of insulin and IGF-1 were tested by ELISA. Prostate tissue of TRAMP mice was used for HE staining.

Results

A total of 17 deaths of TRAMP mice were observed, including 3 (10 %) from the normal diet group, 10 (33.33 %) from the HFD group, and 4 (13.33 %) from Met-HFD group. The mortality rate of TRAMP mice from HFD group was significantly higher than that of normal diet group (P = 0.028), and metformin could moderately decrease the mortality rate by 60.01 % (P = 0.067). Tumor formation rates were not significantly different among the three groups. Levels of glucose, insulin, and IGF-1 tended to increase with TRAMP mice’s age in HFD group. TRAMP mice from HFD group had higher serum insulin and IGF-1 levels. A moderate decrease in IGF-1 was also seen in Met-HFD group.

Conclusions

HFD could promote TRAMP mouse PCa development and progression and metformin had moderate effect of reducing PCa mortality rate with a decrease in serum IGF-1 level.

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Conflict of interest

All authors declare there is no conflict of interest.

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Authors and Affiliations

  1. Department of Urology, Huashan Hospital, Fudan University, 12 WuLuMuQi Middle Road, Shanghai, 200040, People’s Republic of China

    Hua Xu, Meng-bo Hu, Pei-de Bai, Wen-hui Zhu, Qiang Ding & Hao-wen Jiang

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  1. Hua Xu
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  2. Meng-bo Hu
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  3. Pei-de Bai
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  4. Wen-hui Zhu
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  5. Qiang Ding
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  6. Hao-wen Jiang
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Correspondence to Hao-wen Jiang.

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Xu, H., Hu, Mb., Bai, Pd. et al. Will metformin postpone high-fat diet promotion of TRAMP mouse prostate cancer development and progression?. Int Urol Nephrol 46, 2327–2334 (2014). https://doi.org/10.1007/s11255-014-0823-x

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  • DOI: https://doi.org/10.1007/s11255-014-0823-x

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