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MTHFR, MTR and MTRR polymorphisms and risk of chronic kidney disease in Japanese: cross-sectional data from the J-MICC Study

  • Nephrology - Original Paper
  • Published:
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Abstract

Purpose

Chronic kidney disease (CKD) is well known as a strong risk factor for both of end-stage renal disease and cardiovascular disease. To clarify the associations of MTHFR, MTR, and MTRR polymorphisms with the risk of CKD in Japanese, we examined this association among Japanese subjects using cross-sectional data.

Methods

The subjects for this analysis were 3,318 participants consecutively selected from the Japan Multi-institutional Collaborative Cohort (J-MICC) Study. The polymorphisms were genotyped by a multiplex polymerase chain reaction-based Invader assay. Age- and sex-adjusted odds ratio (aOR) of CKD with stage 3–5 was calculated for each genotype.

Results

When those with MTHFR C677T C/C were defined as references, those with MTHFR C677T C/T and T/T demonstrated the aORs for CKD of 1.14 (95 % CI 0.93–1.40) and 1.39 (1.06–1.82), respectively. Marginally significantly decreased risk of CKD with increasing number of MTR A2756G G allele (p = 0.058) was observed. Stratified analyses by plasma folate low (<7.4 ng/ml) or high (≥7.4 ng/ml) suggested significantly higher OR of CKD for those with MTHFR C677T T/T and low serum folate with the aOR of 2.07 (95 % CI 1.30–3.31) compared with that for those with MTHFR C677T T/T and high serum folate.

Conclusions

The present study found a significant association between the subjects with the T/T genotype of MTHFR C677T polymorphism and the elevated risk of CKD, which may suggest the possibility of the risk evaluation and prevention of this potentially life-threatening disease based on genetic traits in the near future.

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Acknowledgments

The authors wish to thank Mr. Kyota Ashikawa and Ms. Tomomi Aoi at the Laboratory of Genotyping Development, Center for Genomic Medicine, RIKEN, for genotyping. The authors also thank Ms. Yoko Mitsuda and Ms. Keiko Shibata at Daiko Medical Center, Nagoya University, for their technical assistance. This study was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas of Cancer (No. 17015018) and Scientific Support Programs for Cancer Research, and Grant-in-Aid for Scientific Research on Innovative Areas (No. 221S0001) from the Japanese Ministry of Education, Culture, Sports, Science and Technology.

Conflict of interest

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Authors and Affiliations

  1. Department of Preventive Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan

    Asahi Hishida, Rieko Okada, Yin Guang, Mariko Naito & Kenji Wakai

  2. Division of Epidemiology and Prevention, Aichi Cancer Center Research Institute, Nagoya, Japan

    Satoyo Hosono & Hideo Tanaka

  3. St. Mary’s College Faculty of Nursing, Kurume, Japan

    Kazuyo Nakamura

  4. Department of Health Science, Shiga University of Medical Science, Otsu, Japan

    Tanvir Chowdhury Turin

  5. Department of Medicine, University of Calgary, Calgary, AB, Canada

    Tanvir Chowdhury Turin

  6. Department of Public Health, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan

    Sadao Suzuki

  7. Department of International Island and Community Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan

    Hideshi Niimura

  8. Division of Epidemiology, Chiba Cancer Center Research Institute, Chiba, Japan

    Haruo Mikami

  9. Department of Preventive Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan

    Jun Otonari

  10. Department of Social Medicine and Cultural Sciences, Kyoto Prefectural University of Medicine, Kyoto, Japan

    Nagato Kuriyama

  11. Department of Preventive Medicine, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan

    Sakurako Katsuura

  12. Center for Genomic Medicine, RIKEN, Yokohama, Japan

    Michiaki Kubo

  13. Young Leaders’ Program, Nagoya University Graduate School of Medicine, Nagoya, Japan

    Nobuyuki Hamajima

Authors
  1. Asahi Hishida
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  2. Rieko Okada
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  3. Yin Guang
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  4. Mariko Naito
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  8. Tanvir Chowdhury Turin
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  15. Michiaki Kubo
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  16. Hideo Tanaka
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  17. Nobuyuki Hamajima
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Corresponding author

Correspondence to Asahi Hishida.

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Hishida, A., Okada, R., Guang, Y. et al. MTHFR, MTR and MTRR polymorphisms and risk of chronic kidney disease in Japanese: cross-sectional data from the J-MICC Study. Int Urol Nephrol 45, 1613–1620 (2013). https://doi.org/10.1007/s11255-013-0432-0

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  • DOI: https://doi.org/10.1007/s11255-013-0432-0

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