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Cytotoxic effects exerted by polyarylsulfone dialyser membranes depend on different sterilization processes

  • Nephrology - Original Paper
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Abstract

Polyarylsulfone group is one of the most important polymeric materials used in the biomedical field, due to its excellent properties, such as good thermal, chemical, and mechanical stability. There are three important polyarylsulfone polymers, all of which have excellent electrical properties: polysulfone (PSu), polyarylsulfone (PAS) and polyarylethersulfone (PAES). All these polymers have excellent creep, radiation and high temperature resistance. In this study, we aimed to determine the effect of three sterilization processes (steam, ethylene oxide and gamma rays) on cytotoxicity of polyarylsulfone dialysis membranes. Ten long-term dialysis patients and ten age-matched healthy controls were enrolled in our study. We analysed (1) serum effect on cultured endothelial cell viability using MTT assay and (2) lipid peroxidation assessed by serum malondialdehyde (MDA) formation at the beginning (T0), the middle (T2) and the end (T4) of haemodialysis (HD) session. Our results clearly showed that steam-sterilized membranes improve endothelial cell viability when compared to ethylene oxide or gamma rays-sterilized ones. Moreover, there is a increased generation of MDA in patients sera during HD session. The serum MDA concentration was about 3, 6 and 10 times higher, respectively, for steam, ethylene oxide and gamma rays sterilization procedures when compared to the MDA amount in healthy subject sera. We concluded that using steam instead of ethylene oxide or gamma rays for sterilization may improve the biocompatibility of polyarylsulfone membranes.

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Abbreviations

PSu:

Polysulfone

PAS:

Polyarylsulfone

PAES:

Polyarylethersulfone

MDA:

Malondialdehyde

HD:

Haemodialysis

ROS:

Reactive Oxygen species

MTT:

3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium-bromide

TBA:

Thiobarbituric acid

References

  1. Iseki K, Tozawa M et al (2003) Determinants of prescribed dialysis dose and survival in a cohort of chronic hemodialysis patients. Clin Exp Nephrol 7:231–237

    Article  PubMed  Google Scholar 

  2. Chan R, Brooks R et al (2009) The effects of kidney-disease-related loss on long-term dialysis patients’ depression and quality of life: positive affect as a mediator. Clin J Am Soc Nephrol 4:160–167

    Article  PubMed  Google Scholar 

  3. Hoenich NA (2007) Membranes for dialysis: can we do without them? Int J Artif Organs 30:964–970

    PubMed  CAS  Google Scholar 

  4. Kummerle W, Heilmann K (1998) Importance of sterilisation procedures for extracorporeal devices. Blood material interaction. A basic guide from polymer science to clinical application. INFA, Glasgow, Krems, pp 100–105

    Google Scholar 

  5. Kawano Y, Logarezzi AJM (1995) X-ray induced degradation of regenerated cellulose membrane films. Polym Degrad Stab 50:125–130

    Article  CAS  Google Scholar 

  6. Rodríguez-Benot A, Santamaría R et al (2002) Sterilization procedures and biocompatibility. Contrib Nephrol 137:138–145

    Article  PubMed  Google Scholar 

  7. Müller TF, Seitz M et al (1998) Biocompatibility differences with respect to the dialyzer sterilization method. Nephron 78:139–142

    Article  PubMed  Google Scholar 

  8. Aucella F, Tetta C et al (2002) Is steam sterilization really making any difference in dialysis-induced cytokine release? Int J Artif Organs 25:832–837

    PubMed  CAS  Google Scholar 

  9. Koken T, Serteser M et al (2004) Changes in serum markers of oxidative stress with varying periods of haemodialysis. Nephrology 9:77–82

    Article  PubMed  Google Scholar 

  10. Droge W (2002) Free radicals in the physiological control of cell function. Physiol Rev 82:47–95

    PubMed  CAS  Google Scholar 

  11. Pawlak K, Pawlak D et al (2007) Impaired renal function and duration of dialysis therapy are associated with oxidative stress and proatherogenic cytokine levels in patients with end-stage renal disease. Clin Biochem 40:81–85

    Article  PubMed  CAS  Google Scholar 

  12. Jackson P, Loughrey CM et al (1995) Effect of hemodialysis on total antioxidant capacity and serum antioxidants in patients with chronic renal failure. Clin Chem 41:1135–1138

    PubMed  CAS  Google Scholar 

  13. Galli F, Canestrari F et al (1999) Pathophysiology of the oxidative stress and its implication in uremia and dialysis. Contrib Nephrol 127:1–31

    Article  PubMed  CAS  Google Scholar 

  14. Becker B, Himmelefarb J et al (1997) Reassessing the cardiac risk profile in chronic hemodialysis patients: a hypothesis on role of oxidant stress and other non-traditional cardiac risk factors. J Am Soc Nephrol 8:475–486

    PubMed  CAS  Google Scholar 

  15. Gutteridge JMC (1995) Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 41:1819–1828

    PubMed  CAS  Google Scholar 

  16. Craddock PR, Fehr J et al (1977) Hemodialysis leukopenia: pulmonary vascular leukostasis resulting from complement activation by dialyser cellophane membrane. J Clin Invest 59:879–888

    Article  PubMed  CAS  Google Scholar 

  17. Bonomini M, Reale M et al (1998) Serum levels of soluble adhesion molecules in chronic renal failure and dialysis patients. Nephron 79:399–407

    Article  PubMed  CAS  Google Scholar 

  18. Amore A, Bonaudo R et al (1995) Enhanced production of nitric oxide by blood dialysis membrane interaction. J Am Soc Nephrol 6:1278–1283

    PubMed  CAS  Google Scholar 

  19. Sun W, Chen T et al (2007) A study on membrane morphology by digital image processing. J Memb Sci 305:93–102

    Article  CAS  Google Scholar 

  20. Edgell CJ, McDonald CC et al (1983) Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc Natl Acad Sci USA 80:3734–3737

    Article  PubMed  CAS  Google Scholar 

  21. Mosman T (1983) Rapid colorimetric assay for cellular growth and survival. Application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63

    Article  Google Scholar 

  22. Donker RB, Asgeirsdóttir SA et al (2005) Plasma factors in severe early-onset preeclampsia do not substantially alter endothelial gene expression in vitro. J Soc Gynecol Investig 12:98–106

    Article  PubMed  CAS  Google Scholar 

  23. Ohkawa H, Ohishi N et al (1979) Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358

    Article  PubMed  CAS  Google Scholar 

  24. Hugue V, Kandoussi M et al (1996) Alteration of the lipid and apolipoprotein contents of lipoprotein (a) in haemodialysis patients. Nephrol Dial Transplant 11:825–829

    PubMed  CAS  Google Scholar 

  25. Ramos R, Martínez-Castelao A (2008) Lipoperoxidation and hemodialysis. Metabolism 57:1369–1374

    Article  PubMed  CAS  Google Scholar 

  26. Barzin J, Feng C et al (2004) Characterization of polyethersulfone hemodialysis membrane by ultrafiltration and atomic force microscopy. J Memb Sci 237:77–85

    Article  CAS  Google Scholar 

  27. Bélanger MC, Marois Y et al (2000) Selection of a polyurethane membrane for the manufacture of ventricles for a totally implantable artificial heart: blood compatibility and biocompatibility studies. Artif Organs 24:879–888

    Article  PubMed  Google Scholar 

  28. Vienken J, Diamantoglou M et al (1999) Artificial dialysis membranes: from concept to large scale production. Am J Nephrol 19:355–362

    Article  PubMed  CAS  Google Scholar 

  29. Silva AC, Rocha JBT et al (2007) Oxidative stress and d-ALA-D activity in chronic renal failure patients. Biomed Pharmacother 61:180–185

    Article  PubMed  Google Scholar 

  30. Voss P, Siems W (2006) Clinical oxidation parameters of aging. Free Radic Res 40:1339–1349

    Article  PubMed  CAS  Google Scholar 

  31. Himmelfarb J, Hakim RA (2003) Oxidative stress in uremia. Curr Opin Nephrol Hypertens 12:593–598

    Article  PubMed  CAS  Google Scholar 

  32. Jaffe JA, Liftman C et al (2005) Frequency of elevated serum aluminium levels in adult dialysis patients. Am J Kidney Dis 46:316–319

    Article  PubMed  Google Scholar 

  33. Santoro A, Ferrari G et al (1996) Ethylene-oxide and steam-sterilised polysulfone membrane in dialysis patients with eosinophilia. Int J Artif Organs 19:329–335

    PubMed  CAS  Google Scholar 

  34. Aucella F, Vigilante M et al (1998) Reduction of mononuclear cytokine production in hemodialysis patients treated with steam-sterilized low-flux polysulphone membranes. Int J Artif Organs 21:210–215

    PubMed  CAS  Google Scholar 

  35. Dastgir M, Peeva LG et al (2005) Stabilization of supported liquid membranes by γ-radiation and their performance in the membrane aromatic recovery system. Ind Eng Chem Res 44:7659–7667

    Article  CAS  Google Scholar 

  36. Vázquez MI, de Lara R et al (2005) Modification of cellophane membranes by γ-radiation: effect of irradiation doses on electrochemical parameters. J Membr Sci 256:202–208

    Google Scholar 

  37. Shintani H, Nakamura A (1989) Analysis of a carcinogen, 4, 4′ methylenedianiline, from thermosetting polyurethane during sterilization. J Anal Toxical 13:354–357

    CAS  Google Scholar 

  38. Vàquez MI, Galàn P et al (2004) Effect of radiation and thermal treatment on structural and transport parameters for cellulose regenerated membranes. Appl Surf Sci 238:415–422

    Article  Google Scholar 

  39. Zundorf J, Kremer S et al (2008) Formation of hydrogen fluoride by gamma and beta sterilisation in medical devices containing perfluoroheptane. Toxicology 243:284–293

    Article  PubMed  Google Scholar 

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Acknowledgements

This research was supported by «Le Ministère Tunisien de l’Enseignement Supérieur, de la Recherche Scientifique et de la Technologie (Laboratoire de Recherche sur les Substances Biologiquement Compatibles: LRSBC) ».

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Authors and Affiliations

  1. Laboratoire de Recherche sur les Substances biologiquement Compatible (LRSBC), faculté de Medecine Dentaire, Monastir, Tunisia

    Emna El Golli-Bennour, Bochra Kouidhi, Mouna Dey, Rabia Younes, Chayma Bouaziz, Chiraz Zaied, Hassen Bacha & Addellatif Achour

  2. Service de néphrologie, hémodialyse et transplantation rénale, CHU Sahloul, Sousse, Tunisia

    Mouna Dey, Rabia Younes & Addellatif Achour

Authors
  1. Emna El Golli-Bennour
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  2. Bochra Kouidhi
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  3. Mouna Dey
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  4. Rabia Younes
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  5. Chayma Bouaziz
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  7. Hassen Bacha
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Correspondence to Hassen Bacha.

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Golli-Bennour, E.E., Kouidhi, B., Dey, M. et al. Cytotoxic effects exerted by polyarylsulfone dialyser membranes depend on different sterilization processes. Int Urol Nephrol 43, 483–490 (2011). https://doi.org/10.1007/s11255-009-9653-7

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