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Protective effect of Antrodia Camphorata on bladder ischemia/reperfusion injury

  • Urology - Original Paper
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Abstract

Introduction

Obstructive bladder dysfunction is directly related to ischemia/reperfusion injury characterized by damage to nerves, synapses and smooth muscle cells within the bladder wall. Antrodia Camphorata (AC) has significant antioxidant, antiinflammatory and cell-cycle inhibition properties. The specific aim of this study was to evaluate whether orally administered AC can protect rabbit bladders from the progressive dysfunctions induced by bilateral ischemia/reperfusion (I/R).

Methods

Twenty-four male NZW rabbits were separated into 4 groups of 6 animals each. Rabbits in groups 1 and 2 were fed Antrodia Camphorata (AC) suspensions; those in groups 3 and 4 received vehicle. Each rabbit in groups 2 and 4 were subjected to in vivo bilateral ischemia for 2 h and then allowed to recover for 1 week. The rabbits in groups 1 and 3 received sham operation and served as control groups. Cystometry, contractile responses to field stimulation, carbachol, ATP and KCl were determined. Biochemical and immuno-histochemical studies were also performed.

Results

I/R resulted in decreased compliance, decreased contractile responses, decreased nerve density, and increased apoptosis. AC pretreatment of rabbits subjected to I/R significantly protected the bladder from all contractile, biochemical, and structural dysfunctions resulting in significantly improved bladder.

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Acknowledgments

This material is based upon work supported in part by a generous grant from the Wellshine Biotech Company, Taipei, Taiwan; in part by the Office of Research and Development Medical Research Service, Department of Veteran’s Affairs; and in part by NIH grant RO-1-DK 067114.

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Authors and Affiliations

  1. Department of Urology, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan

    Yung-Shun Juan

  2. Graduate Institute of Medicine, Department of Urology, Kaohsiung Medical University, Kaohsiung, Taiwan

    Yung-Shun Juan & Shu Mien Chuang

  3. Albany College of Pharmacy, 106 New Scotland Avenue, Albany, NY, 12208, USA

    Alpha D. Lin, Robert E. Leggett & Robert M. Levin

  4. Albany Medical College, Albany, NY, USA

    Anita Mannikarottu & Robert M. Levin

  5. Stratton Veterans Affairs Medical Center, Albany, NY, USA

    Suning Li, Catherine Schuler & Robert M. Levin

  6. First Affiliated Hospital of Zhengzhou University, Henan, China

    Suning Li & Catherine Schuler

  7. Taichung Poh-Ai Hospital, Taichung, Taiwan

    Alpha D. Lin

  8. Wellshine Biotech Company, Taipei, Taiwan

    Lan Chang-Chou

Authors
  1. Yung-Shun Juan
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  2. Anita Mannikarottu
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  3. Shu Mien Chuang
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  4. Suning Li
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  5. Alpha D. Lin
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  6. Lan Chang-Chou
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  7. Catherine Schuler
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  8. Robert E. Leggett
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  9. Robert M. Levin
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Corresponding author

Correspondence to Robert M. Levin.

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Juan, YS., Mannikarottu, A., Chuang, S.M. et al. Protective effect of Antrodia Camphorata on bladder ischemia/reperfusion injury. Int Urol Nephrol 42, 637–645 (2010). https://doi.org/10.1007/s11255-009-9642-x

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  • DOI: https://doi.org/10.1007/s11255-009-9642-x

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