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The first molecular detection of Anaplasma capra in domestic ruminants in the central part of Turkey, with genetic diversity and genotyping of Anaplasma capra

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Abstract

Tick-borne diseases have been an increasing threat to human and animal health all over the world. Anaplasmosis is one of the emerging tick-borne diseases and has zoonotic potential. A new novel species, which was detected in China in 2010–2012 and provisionally named Anaplasma capra in 2015, causes zoonotic infections and infects many different animal species. In this study, we investigated the presence of A. capra in domestic ruminants from Turkey. A total of 468 blood samples (cattle, sheep, and goat) were examined by the gltA gene-specific nested polymerase chain reaction, revealing the presence of A. capra in six samples (1.28%): one of them from cattle (0.41%) and the other five from sheep (3.22%). According to DNA sequences results of the gltA gene, A. capra isolates identified in the present study were shown high nucleotide similarity with A. capra isolates detected from different hosts. However, the nucleotide differences were detected in the same nucleotide positions between A. capra isolates. For this reason, we thought that at least two different A. capra genotypes could be circulating in the world. As a result, it is seen that A. capra, which was determined to be a new species with zoonotic potential, was revealed in European and Asian countries and in different hosts. In order to raise awareness about human anaplasmosis infections, it is important to reveal the prevalence of the species in the world. The emergence of A. capra in Turkey reveals the need for a re-evaluation of the human and animal health risk analysis in terms of anaplasmosis.

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Acknowledgements

The authors would like to thank all veterinarians and technicians for their kind help during sample collection.

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Authors and Affiliations

  1. Department of Parasitology, Faculty of Veterinary Medicine, University of Sivas Cumhuriyet, 58140, Sivas, Turkey

    Kursat Altay, Ufuk Erol & Omer Faruk Sahin

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  1. Kursat Altay
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  2. Ufuk Erol
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  3. Omer Faruk Sahin
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Contributions

Kursat Altay (DVM, PhD, Prof.): Conceptualization, field work, methodology, validation, formal analysis, supervision, writing—original draft preparation, reviewing, and editing

Ufuk EROL (DVM, PhD, Assist. Prof.): Conceptualization, field work, methodology, validation, data curation, formal analysis, writing—original draft preparation

Omer Faruk SAHİN (DVM, Res. Assist.): Field work, data curation, methodology, formal analysis

Corresponding author

Correspondence to Kursat Altay.

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Ethics approval

All procedures performed in studies involving animals were in accordance with the ethical standards approved by the Sivas Cumhuriyet University Animal Experiments Local Ethics Committee (Approval number: 65202830–050.04.04–573).

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The consent of all animal owners was sorted before this study was carried out.

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Altay, K., Erol, U. & Sahin, O.F. The first molecular detection of Anaplasma capra in domestic ruminants in the central part of Turkey, with genetic diversity and genotyping of Anaplasma capra. Trop Anim Health Prod 54, 129 (2022). https://doi.org/10.1007/s11250-022-03125-7

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