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The effects of vitamin C and methionine hydroxy analog supplementation on performance, blood parameters, liver enzymes, thyroid hormones, antioxidant activity of blood plasma, intestine morphology, and HSP70 gene expression of broilers under heat stress

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Abstract

This experiment was performed to evaluate the effect of vitamin C and methionine hydroxy analog (MHA) on performance, blood parameters, liver enzymes, activity of selected hormones, antioxidant activity of blood plasma, intestine morphology, and HSP70 gene expression in broilers under heat stress. Four hundred 1-day-old male chickens of commercial Ross 308 strain were used in a completely randomized design with 4 treatments and 5 replicates of 20 chickens each. The experimental treatments were as follows: (1) base diet based on maize soybean (the control), (2) base diet with 0.2% vitamin C, (3) base diet with 0.46, 0.36, and, 0.32% methionine hydroxy analog, in the starter, grower, and finisher periods, respectively, and (4) base diet with a mixture of 0.2% vitamin C and 0.46, 0.36, and 0.32% of methionine hydroxy analog in the starter, grower, and finisher periods respectively. Although numerically higher than control, the effect of the experimental diets on performance was not significant across the experimental period (P>0.05). Glucose and LDL concentrations under all experimental treatments were significantly reduced compared to the control (P<0.05). The concentrations of liver enzymes (AST and ALT) and antioxidant activity (SOD, GPx, and MDA) of broilers were significantly different from the control under combined MHA and vitamin C supplementation (P<0.05). All experimental diets led to significantly increased T4 concentrations, compared to control (P<0.05). The length and width of intestinal villi of broilers were significantly increased in all of the experimental treatments compared to the control (P<0.05). The difference in HSP70 gene expression was significantly reduced in all of the treatments compared to the control (P<0.05). The results of this experiment showed that the use of vitamin C and methionine hydroxy analog (MHA) can be effective in reducing the negative effects of heat stress.

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Funding

This research was done with the personal funding of the authors.

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Authors and Affiliations

  1. Department of Animal Science, Karaj Branch, Islamic Azad University, Karaj, Iran

    Mahsa Erfani, Nima Eila, Abolfazl Zarei & Alireza Noshary

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  1. Mahsa Erfani
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  2. Nima Eila
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Contributions

In the process of writing the article, Mahsa Erfani was responsible for the provision, integration, and writing of the article data, Nima Eila was responsible for the research conception and design, Abolfazl Zarei was the article reviewer, and Alireza Noshary took a part in data collection in the trial process.

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Correspondence to Nima Eila.

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The experimental protocol was ratified by the Animal Ethic Committee of the Islamic Azad University, Karaj, Iran, and the experiment was performed with respect to the International Guidelines for research involving animals (Directive 2010/63/EU).

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Erfani, M., Eila, N., Zarei, A. et al. The effects of vitamin C and methionine hydroxy analog supplementation on performance, blood parameters, liver enzymes, thyroid hormones, antioxidant activity of blood plasma, intestine morphology, and HSP70 gene expression of broilers under heat stress. Trop Anim Health Prod 53, 296 (2021). https://doi.org/10.1007/s11250-021-02738-8

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