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Maternal supplementation with fish oil modulates inflammation-related MicroRNAs and genes in suckling lambs

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Abstract

Dietary n-3 long-chain fatty acids (n-3 LCFA) have been shown to modify lipid metabolism and immune function. The objective of this study was to evaluate the effect of periparturient fish oil (FO) supplementation on the inflammation and metabolic health of ewes and their lambs at a molecular level. Prepartum ewes were fed control diet (CON, n = 12) or CON supplemented with 2% DM of calcium soap of FO (n = 12) from 28 days before until 21 days after parturition. The ewes were evaluated for plasma metabolites and milk composition. The experiment was followed by analyzing the relative transcript abundance of circulating microRNAs (miRNAs) in plasma and targeted miRNA/mRNA expression in peripheral blood mononuclear cells (PBMCs) in both ewes and lambs. FO treatment decreased prepartum feed intake (1812 ± 35 vs 1674 ± 33 g/day, P < 0.01), whereas the influence on plasma metabolites was negligible. Dietary FO supplementation decreased milk fat percentage (8.82 ± 0.49 vs 7.03 ± 0.45, P = 0.02) and reduced milk n-6/n-3 (P < 0.05). Also, it altered the expression of plasma-circulating miRNAs in both ewe and lamb (P < 0.05). Furthermore, maternal nutrition of FO downregulated the relative expression of miR-33a and miR-146b and transcript abundance of genes IL-1β (0.41-fold) and NF-κB (0.25-fold) in lambs’ PBMC. In conclusion, results showed that FO supplementation starting antepartum affects milk composition and circulating miRNA in dams and the inflammatory markers in lambs delivered by the supplemented ewes. These may provide a strategy to maintain immune balance during gestation and develop the immune system in lambs.

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Acknowledgments

The authors thank the members of their own laboratories for their helpful assistance.

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This work was supported by a grant from the University of Tehran, Tehran, Iran.

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  1. Department of Animal and Poultry Science, Aburaihan Campus, University of Tehran, P.O. Box 3391653755, Pakdasht, Tehran, Iran

    Arash Veshkini, Abdollah Mohammadi-Sangcheshmeh, Ali A. Alamouti & Abdolreza Salehi

  2. Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran

    Fatemeh Kouhkan

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  1. Arash Veshkini
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  2. Abdollah Mohammadi-Sangcheshmeh
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  3. Ali A. Alamouti
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Correspondence to Ali A. Alamouti.

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The experiments in sheep were in accordance with the ethical standards of the stem cell research center and institutional and/or national research committee of the University of Tehran, Iran. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Veshkini, A., Mohammadi-Sangcheshmeh, A., Alamouti, A.A. et al. Maternal supplementation with fish oil modulates inflammation-related MicroRNAs and genes in suckling lambs. Trop Anim Health Prod 52, 1561–1572 (2020). https://doi.org/10.1007/s11250-019-02157-w

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