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Immunoprotective role of LaSota vaccine under immunosuppressive conditions in chicken challenged with velogenic avian avulavirus-1

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Abstract

The first objective of the present study was to evaluate if the antibodies induced by the live LaSota and killed Newcastle disease (sub-genotype VIIi) vaccines protect the chickens against exposure with pathogenic avian avulavirus-1 (AAvV-1) of chicken and/or pigeon origins. The second objective was to study the effect of vaccines on stressed birds (dexamethasone, aflatoxin, and heat stressed) with respect to antibody production and protection against pathogenic AAvV-1 challenge. Sixty-one-day-old Hubbard chickens were divided into six groups (gA–gF) with ten animals each. All the groups received LaSota (105 EID50, 0.1 ml per chick) on days 7 and 27 via eye drop and one intramuscular injection of a killed vaccine (sub-genotype VIIi) (107.5 EID50, 1 ml) on day 18, except the control birds received the PBS only. Moreover, group gC-DEX received dexamethasone intramuscularly at a dose rate of 1-mg/kg body weight daily; gD-AFLA had received aflatoxin as oral gavage at a dose rate of 30 ppb daily, and gE-HEAT was kept under heat stressed (38 °C) till challenged. All the groups were challenged with AAvV-1 strain of chicken origin of sub-genotype VIIi, except the group gA-pigeon was challenged with pigeon-origin strain (sub-genotype VIm). The result showed that the gA-pigeon and gB-chicken vaccinate showed 100% and 80% protection. The immunosuppressive birds produced low pre-challenge HI titer, and protection was observed at 40%, 50%, and 70% in gC-DEX, gD-AFLA, and gE-HEAT, respectively. Our findings suggest the stress factors such as aflatoxin in the feed and dexamethasone are immunosuppressive in nature and suppress the immune response and its associated protective role during infection.

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Author notes

  1. Iqra Rauf and Abdul Wajid contributed equally to this work.

Authors and Affiliations

  1. Department of Microbiology, University of Veterinary and Animal Science Lahore, Lahore, Pakistan

    Iqra Rauf, Irshad Hussain & Muhammad Asad Ali

  2. Department of Biotechnology, Virtual University of Pakistan, 1-Davis Road Lahore, Lahore, Pakistan

    Abdul Wajid

  3. Department of Molecular Biology, Virtual University of Pakistan, 1-Davis Road Lahore, Lahore, Pakistan

    Safa Ather

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  1. Iqra Rauf
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  2. Abdul Wajid
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Correspondence to Abdul Wajid.

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Rauf, I., Wajid, A., Hussain, I. et al. Immunoprotective role of LaSota vaccine under immunosuppressive conditions in chicken challenged with velogenic avian avulavirus-1. Trop Anim Health Prod 51, 1357–1365 (2019). https://doi.org/10.1007/s11250-019-01814-4

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