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Serological and molecular survey of sheep infected with Mycoplasma ovipneumoniae in Xinjiang, China

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Abstract

Mycoplasma pneumonia is one of the most important infectious diseases that threaten sheep production. In order to investigate the epidemic status of Mycoplasma ovipneumoniae infection in sheep, indirect hemagglutination assay was used to analyze 1679 serum samples collected from four different breeds of sheep (Kazak sheep, Hu sheep, Merino sheep, and Duolang sheep) in six regions in Xinjiang between 2012 and 2014. One thousand one hundred sixty-nine sheep nasal swabs and 180 lungs were PCR analyzed. The results showed that the average positive rates of the serum samples were 17.75 %. The positive rates were between 9.76 and 30.61 % in the four breeds. Among them, the Hu sheep had a significantly higher rate than other breeds (P < 0.05). The average positive rates of nasal swabs and lungs were 10.18 and 28.89 %, respectively. Based on the phylogenetic trees of 16S RNA gene, the isolates were closest to those strains isolated from inland areas of China, indicating that these epidemic isolates came from the trans-province introductions. Our survey suggests that quarantine is necessary for sheep imported from inland, and effective immunization should be implemented in sheep susceptible to M. ovipneumoniae in Xinjiang, China.

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Acknowledgments

We thank the field staff from Tacheng, Yili, Altay, Shihezi, Changji, and Akesu who provided the samples for this study. This work was supported by the International Science & Technology Cooperation Program of China (no. 2014DFR31310), National Natural Science Foundation of China (31260601, 31360596, 31460654).

Conflict of interest

The authors declare that they have no competing interests.

Compliance with ethical standards

The experiments were carried out in accordance with the guidelines issued by the Ethical Committee of Shihezi University.

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Authors and Affiliations

  1. College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China

    Chen Cheng, Qiao Jun, Meng Qingling, Hu Zhengxiang, Ma Yu & Chen Chuangfu

  2. State Key Lab of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China

    Cai Xuepeng

  3. Center for Animal Disease Prevention and Control, Tacheng, 834700, Xinjiang, China

    Cheng Zibing & Zhang Jinsheng

  4. Center for Animal Disease Prevention and Control, Changji, 831100, Xinjiang, China

    Zhang Zaichao

  5. Center for Animal Disease Prevention and Control, Xinjiang, 830000, China

    Cai Kuojun

Authors
  1. Chen Cheng
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  2. Qiao Jun
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  3. Meng Qingling
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  4. Hu Zhengxiang
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Correspondence to Qiao Jun.

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Cheng, C., Jun, Q., Qingling, M. et al. Serological and molecular survey of sheep infected with Mycoplasma ovipneumoniae in Xinjiang, China. Trop Anim Health Prod 47, 1641–1647 (2015). https://doi.org/10.1007/s11250-015-0908-2

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