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Evaluation of hepatitis E virus infection between different production systems of pigs in Brazil

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Abstract

The objectives of this study were to (1) investigate the occurrence of hepatitis E virus (HEV) in pigs from large-scale and family-scale farms, (2) genetically characterize the strains isolated, and (3) study the pathogenesis of swine HEV infection via immunohistochemistry. A total of 50 pigs from 10 farms in Mato Grosso State, Brazil were divided according to type of production system into either large-scale farms (n = 5) or family-scale farms (n = 5). Samples of liver, gallbladder, small and large intestines, bile, and feces from the pigs were analyzed by nested PCR with primers targeting the ORF2 region of HEV and by immunohistochemistry. Of the eight HEV-positive samples from pigs of family-scale farms, phylogenetic analysis revealed that seven of the swine HEV isolates clustered with subtype 3b of genotype 3 and one isolate was categorized with subtype 3 f. The HEV antigen was detected mainly in the small intestine samples from family-scale farms, suggesting an early stage HEV infection. HEV was not detected in the samples of pigs from large-scale farms, reinforcing the need for additional studies to evaluate the risk of transmission of HEV to humans from pigs from family-scale farms in Mato Grosso State.

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Acknowledgments

This work was supported by Coordenação de aperfeiçoamento de pessoal em nível superior (CAPES).

Conflict of interest

The authors report no conflicts of interest.

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Authors and Affiliations

  1. Laboratory of Veterinary Pathology, Federal University of Mato Grosso (UFMT), Av. Fernando Corrêa da Costa, 2367. Bairro Boa Esperança, CEP 78069-900, Cuiabá, Mato Grosso, Brazil

    Marconni Victor da Costa Lana, Raquel Aparecida Sales da Cruz, Letícya Lerner Lopes, Gustavo Sousa Silva, Marcos de Almeida Souza, Edson Moleta Colodel & Caroline Argenta Pescador

  2. Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, P.O. Box 6001, 86051-990, Paraná, Brazil

    Noemi Rovaris Gardinali & Amauri Alcindo Alfieri

  3. Department of Basic Science and Animal Production, Federal University of Mato Grosso (UFMT), Av. Fernando Corrêa da Costa, 2367. Bairro Boa Esperança, CEP 78069-900, Cuiabá, Mato Grosso, Brazil

    João Garcia Caramori Júnior

  4. Department of Statistic, Federal University of Mato Grosso (UFMT), Av. Fernando Corrêa da Costa, 2367. Bairro Boa Esperança, CEP 78069-900, Cuiabá, Mato Grosso, Brazil

    Anderson Castro Soares de Oliveira

Authors
  1. Marconni Victor da Costa Lana
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  2. Noemi Rovaris Gardinali
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  3. Raquel Aparecida Sales da Cruz
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  4. Letícya Lerner Lopes
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  5. Gustavo Sousa Silva
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  6. João Garcia Caramori Júnior
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  7. Anderson Castro Soares de Oliveira
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  8. Marcos de Almeida Souza
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  9. Edson Moleta Colodel
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  10. Amauri Alcindo Alfieri
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  11. Caroline Argenta Pescador
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Corresponding author

Correspondence to Caroline Argenta Pescador.

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da Costa Lana, M.V., Gardinali, N.R., da Cruz, R.A.S. et al. Evaluation of hepatitis E virus infection between different production systems of pigs in Brazil. Trop Anim Health Prod 46, 399–404 (2014). https://doi.org/10.1007/s11250-013-0503-3

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  • DOI: https://doi.org/10.1007/s11250-013-0503-3

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