Abstract
The identification of Leptospira clinical isolates through genotyping and serotyping, besides the recognition of its reservoirs, are important tools for understanding the epidemiology of leptospirosis, and they are also keys for identifying new species and serovars. Fourteen clinical isolates from animals were characterized by means of single enzyme amplified length polymorphism, variable number of tandem repeat analysis, pulsed field gel electrophoresis, and serotyping. All isolates were identified as Leptospira interrogans, serovar Canicola. Infections by this serovar occur in urban regions, where dogs represent the main maintenance hosts, whereas bovine and swine may act as reservoirs of serovar Canicola in rural areas. Both urban and rural aspects of leptospirosis, and the role of domestic animals as maintenance hosts, cannot be neglected in developing and developed countries.
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Acknowledgments
We would like to express our gratitude for the help of Dr Paul Levett in performing the PFGE reactions. This study was supported by FAPESP-Fundação de Amparo a Pesquisa do Estado de São Paulo—research projects 2009/52925-2 and 2011/18290-0.
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An erratum to this article is available at http://dx.doi.org/10.1007/s11250-014-0555-z.
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Miraglia, F., de Morais, Z.M., Dellagostin, O.A. et al. Molecular and serological characterization of Leptospira interrogans serovar Canicola isolated from dogs, swine, and bovine in Brazil. Trop Anim Health Prod 45, 117–121 (2012). https://doi.org/10.1007/s11250-012-0181-6
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DOI: https://doi.org/10.1007/s11250-012-0181-6