Abstract
Brazil represents the greatest beef producer among tropical countries, and the major obstacle for meat international trade is sanitary problems especially closely related to viral encephalitis. The goal of this study was to estimate the accuracy of the glycol and US9 gene-based polymerase chain reactions (PCRs) for the detection of bovine Herpesvirus type 5 (BoHV-5) from decomposed brain samples (n = 95). For this purpose, a latent-class (Bayesian) approach was used. Sensitivity (Se) was estimated to be 70% (95% probability interval, 40–80) and specificity (Sp) 100% in the statistical analysis for both PCRs used. Accordingly, a minimum of ≥40% of the calves was estimated to harbor BoHV-5 DNA even after 72 h of decomposition at room temperature. It was concluded that US9 gene-based PCR could also be considered a cost-effective alternative in sanitary programmers. However, given the importance of veterinary diagnoses, PCR-positive samples should be further confirmed through in vitro isolation and/or sequencing.
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References
Alegre, M., Nanni, M. and Fondevila, N, 2001. Development of a multiplex polymerase chain reaction for the differentiation for bovine Herpesvirus 1 and 5. Journal Veterinary Medicine B, 48, 613–621.
Cardoso, T.C., Ferrari, H.F., Luvizotto, M.C.R. and Arns, C.W, 2007. Bio-safety technology in production of Bovine Herpesvirus type 5 (BoHV-5) using an alternative serum-free medium. American Journal Biochemistry Biotechnology, 3, 125–130.
Claus, M.P., Afieri, A.F., Folgueras-Flatschart, A.V. and Alfieri, A.A, 2005. Rapid detection and differentiation of bovine herpesvirus 1 and 5 glycoprotein C gene in clinical specimens by multiplex-PCR. Journal Virological Methods, 128, 183–188.
Enøe, C., Georgiadis, M.P. and Johnson, W.O, 2000. Estimation of sensitivity and specificity of diagnostic tests and disease prevalence when the true disease state is unknown. Preventive Veterinary Medicine, 45, 61–81.
Greiner, M. and Gardner, I.A, 2000. Epidemiologic issues in the validation of veterinary diagnostic tests. Preventive Veterinary Medicine, 45, 3–22.
Johnson, W.O., Gastwirth, J.L. and Pearson, L.M, 2001. Screening without a “Gold Standard”: The Hui-Walter paradigm revisited. American Journal Epidemiology, 153, 921–923.
Joseph, L., Gyorkos, T.W. and Coupal, L, 1995. Bayesian estimation of disease prevalence and the parameters of diagnostic tests in the absence of a gold standard. American Journal Epidemiology, 141, 263–272.
Nandi, S., Kumar, M., Manohar, M. and Chauhan, R. S, 2009. Bovine herpes virus infections in cattle. Animal Health Research Reviews, 10, 85–98.
Quinn, F.D., 1997. Sample preparation for nucleic acid amplification. In: Lee, H., Morse, S., Olsvik, Ø (eds) Nucleic Acid Amplification Technologies. Eaton, Cambridge, pp. 49–60.
Wilson, I.G, 1997. Inhibition and facilitation of nucleic acid amplifications. Applied Environment Microbiology, 63, 3741–4375.
Wrathall, A.E., Simmons, H.A. and Van Soom, A., 2006. Evaluation of risks of viral transmission of recipients of bovine embryos arising from fertilization with virus-infected semen. Theriogenology, 65, 247–274.
Acknowledgments
This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo, Brazil. TC Cardoso is a fellowship of CNPq.
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Cardoso, T.C., Antello, T.F., Vieira, F.V. et al. Estimation of the diagnostic accuracy of the glyco-C and US9 gene-based polymerase chain reaction technique for the detection of bovine Herpesvirus type 5 DNA in decomposed brain suspension from a slaughter house using Bayesian analysis, Brazil. Trop Anim Health Prod 44, 379–382 (2012). https://doi.org/10.1007/s11250-011-9929-7
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DOI: https://doi.org/10.1007/s11250-011-9929-7