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Ovicidal activity of seven Pochonia chlamydosporia fungal isolates on Ascaris suum eggs

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Abstract

The ovicidal effect of the nematophagous fungus Pochonia chlamydosporia on eggs of Ascaris suum was tested under laboratory conditions. A. suum eggs were plated on 2% water–agar with seven fungal isolates (Isol. 5, Isol. 31, Isol. 1, VC1, Isol. 12, Isol. 22 and VC4) and control without fungus. After 5, 7, 10, 14, 15 and 21 days of incubation, approximately 100 eggs were removed from the plates and classified according to the following parameters: type 1, biochemical and physiological effect without morphological damage to the eggshell, type 2, lytic effect with morphological alteration of the eggshell and embryo and type 3, lytic effect with morphological alteration of eggshell and embryo showing hyphal penetration and internal egg colonization. The isolates effectively destroyed A. suum eggs and all types of effects were observed during the experiment. There was no variation in ovicidal capacity (type 3 effect) among the isolates (p > 0.05) throughout the experiment. After 21 days, isolate 5 showed the highest percentages of type 3 effect (58.33%). The results indicated that P. chlamydosporia (Isol. 5, Isol. 31, Isol. 1, VC1, Isol. 12, Isol. 22 and VC4) can destroy A. suum eggs and is, therefore, a potential biological control agent of nematodes.

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Authors and Affiliations

  1. Departamento de Veterinária, Universidade Federal de Viçosa, Rua PhRolfs s/n, Viçosa, Minas Gerais, Cep 36570000, Brazil

    Sebastião R. Ferreira, Jackson V. Araújo, Fabio R. Braga, Juliana M. Araujo, Rogério O. Carvalho, André R. Silva & Luiza N. Frassy

  2. Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Cep 36570000, Brazil

    Leandro G. Freitas

Authors
  1. Sebastião R. Ferreira
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  2. Jackson V. Araújo
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  3. Fabio R. Braga
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  4. Juliana M. Araujo
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  5. Rogério O. Carvalho
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  6. André R. Silva
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  7. Luiza N. Frassy
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  8. Leandro G. Freitas
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Correspondence to Fabio R. Braga.

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Ferreira, S.R., Araújo, J.V., Braga, F.R. et al. Ovicidal activity of seven Pochonia chlamydosporia fungal isolates on Ascaris suum eggs. Trop Anim Health Prod 43, 639–642 (2011). https://doi.org/10.1007/s11250-010-9744-6

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  • DOI: https://doi.org/10.1007/s11250-010-9744-6

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