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Prevalence and molecular diagnosis of Trypanosoma evansi in Nili-Ravi buffalo (Bubalus bubalis) in different districts of Punjab (Pakistan)

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Abstract

The prevalence of Trypanosoma evansi was investigated in 1,250 Nili-Ravi buffaloes of mixed age and sex by polymerase chain reaction (PCR) for the first time in Pakistan. DNA of the trypanosomes was isolated with TRIREAGENT®. The assay was employed using primers ESAG 6/7, specific for a 237-bp fragment from T. evansi genomic DNA. The samples were screened for the presence of T. evansi also by stained thin smear. Forty-four (3.5%) samples were positive by microscopy, while 97 (7.7%) samples were identified by PCR, indicating the high sensitivity of PCR for surveying the disease in epidemiological studies.

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Acknowledgments

The authors gratefully acknowledge the financial support of the government of Punjab for providing necessary funds under the project “Up-gradation of lab facilities at LPRI Bahadurnagar Okara”.

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Authors and Affiliations

  1. Livestock Production Research Institute Bahadurnagar, Okara, Punjab, Pakistan

    Waseem Shahzad, Ashfaq Ahmad & Mohammad Iqbal

  2. Veterinary Research Institute, Lahore, Pakistan

    Rashid Munir

  3. University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan

    Mohammad S. Khan, Mansur D. Ahmad & Mohammad Ijaz

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  1. Waseem Shahzad
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  2. Rashid Munir
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  3. Mohammad S. Khan
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  4. Mansur D. Ahmad
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  5. Mohammad Ijaz
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  6. Ashfaq Ahmad
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  7. Mohammad Iqbal
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Correspondence to Waseem Shahzad.

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Shahzad, W., Munir, R., Khan, M.S. et al. Prevalence and molecular diagnosis of Trypanosoma evansi in Nili-Ravi buffalo (Bubalus bubalis) in different districts of Punjab (Pakistan). Trop Anim Health Prod 42, 1597–1599 (2010). https://doi.org/10.1007/s11250-010-9616-0

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  • DOI: https://doi.org/10.1007/s11250-010-9616-0

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