Abstract
The aim of this work was to determine the influence of different processing procedures and preparations on the viability and infectivity of Trichinella spiralis ML. The muscles of limbs tongue and masseters of pigs experimentally infected were collected, splitted to pieces, and pooled. Five batches were used for the following processing procedures: (1) seasoning with “adobo”, commercially acquired chilli and several other spices, (2) “wet-curing” by immersion of meat pieces in 3% brine during 24 hours, (3) cold storage without any further processing or preparation, (4) freezing to -20°C and, (5) drying for 24 hours at 60°C. Samples were stored at 4°C for 15, 45, 60, 75, 90, 105 or 266 days after preparation. At the last-mentioned dates, ML were recovered and used to determine the reproductive capacity by infecting naïve mice. The state of meat conservation or spoilage respectively was tested by visual and tactile examination. In samples treated by freezing or drying no motile larvae were found after artificial digestion and, following inoculation of mice with larvae recovered from these groups, no ML were founded after 40 days of infection. After the artificial digestion of the cold stored samples, the ones seasoned with “adobo” and “wet-cured”, a number of motile ML were consistently obtained. Initial reproductive capacity index was as of 80±0.5, then rates decreased to 60 – 70 between days 15 and 105 PT and dropped to 40±6.7 at day 266 for seasoned, 33±2.7 for cold-stored and 33±2.5 for cured samples. The influence of storage time (p=0.000005; factorial ANOVA) but not for processing procedure (p=0.724; factorial ANOVA) were statistically significant. The sensorial examination of the meat samples showed severe changes caused by spoilage in odour, texture and colour from day 45 of storage. Data reported from this trial proves that curing or flavoring do not inactivate the Trichinella Mexican strain, although cold storage for more than three months led to a partial decrease of the reproductive capacity. Freezing and drying seemed to be effective measures to eliminate the ML infectivity.
Abbreviations
- ML:
-
Trichinella spiralis muscle larvae
- DPI:
-
day post infection
- DPT:
-
day post treatment
- RCI:
-
reproductive capacity index
References
Blair, L.S., 1983. Laboratory techniques. In: W.C. Campbell (ed). Trichinella and trichinosis, (Plenum Press, New York), 563–568
de-la-Rosa, J.-L., Álvarez, N. and Gómez-Priego, A., 2007. Study of the reproductive capacity of Trichinella spiralis recovered from experimentally infected mice under-dosed with albendazole or mebendazole. Tropical Biomedicine, 24(2), 93–97.
Despommier, D.D., 1971. Immunogenicity of the newborn larva of Trichinella spiralis. The Journal of Parasitology, 57(3): 531–534. doi:10.2307/3277908
Gracey, J.F., Collins, D.S. and Huey, R.J. 1999. Meat Hygiene. W.B. Saunders, London, 10th edition.
Hill, D.E., Forbes, L., Gajadhar, A.A. and Gamble, H.R., 2007. Viability and infectivity of Trichinella spiralis muscle larvae in frozen horse tissue. Veterinary Parasitology, 146(1–2), 102–106. doi:10.1016/j.vetpar.2007.02.001
Malakauskas, A. and Kapel, C.M., 2003. Tolerance to low temperatures of domestic and sylvatic Trichinella spp. in rat muscle tissue. Journal of Parasitology, 89(4), 744–748. doi:10.1645/GE-2969.1
Martinez-Fernandez, A.R., Arribas, B. and Bolás, F., 1997. Freezing resistance of Spanish Trichinella isolates (T. spiralis and T. britovi). In: G. Ortega-Pierres, R. Gamble, F. van Knapen and D. Wakelin (eds). Proceedings of the Ninth International Conference on Trichinellosis, Mexico City, 1996, (International Committee on Trichinellosis; Occasional Publication), 99–105.
Meza, E., Garcia, E., Alvarado, M., Letechepia, M. and Moreno, A., 1997. Evaluation of the effectiveness of temperature on the viability of Trichinella spiralis infective larvae. In: G. Ortega-Pierres, R. Gamble, F. van Knapen and D. Wakelin (eds). Proceedings of the Ninth International Conference on Trichinellosis, Mexico City, 1996, (International Committee on Trichinellosis; Occasional Publication), 93–98.
Norma Oficial Mexicana NOM-062-ZOO-1999. Especificaciones técnicas para la producción, cuidado y uso de los animales de laboratorio.
Norma Oficial Mexicana NOM-194-SSA1-2004. Productos y servicios. Especificaciones sanitarias en los establecimientos dedicados al sacrificio y faenado de animales para abasto, almacenamiento, transporte y expendio. Especificaciones sanitarias.
Oivanen, L., Mikkonen, T., Haltia, L., Karhula, H., Saloniemi, H. and Sukura, A., 2002. Persistence of Trichinella spiralis in rat carcasses experimentally mixed in different feed. Acta Veterinaria Scandinavica, 43(4): 203–210. doi:10.1186/1751-0147-43-203
Ortega-Pierres, M.G., Arriaga, C. and Yepez-Mulia, L., 2000. Epidemiology of trichinellosis in Mexico, Central and South America. Veterinary Parasitology, 93(3–4), 201–225 doi:10.1016/S0304-4017(00)00342-3
Pozio, E., Kapel, C.M.O., Gajadhar, A.A., Boireau, P., Dupouy-Camet, J. and Gamble, H.R., 2006. Trichinella in pork: current knowledge on the suitability of freezing as a public health measure. Eurosurveillance weekly releases, 11(11), 277–278.
Pozio, E. and Murrell, K.D., 2006. Systematic and epidemiology of Trichinella. Advances in Parasitology, 63, 367–439. doi:10.1016/S0065-308X(06)63005-4
Quiroz, R.H. and Bañuelos, P., 1972. Viabilidad de Trichinella spiralis en el chorizo. Veterinaria México, 3, 3–4.
Quiroz, R.H. and Landeros, R.R., 1998. Valoración del tiempo de sobrevivencia de larvas de Trichinella spiralis en chorizo de cerdo. Revista Mexicana de Parasitología, 1(1), 22–24.
Ramírez-Melgar, C., Gómez-Priego, A., de-la-Rosa, J.-L., 2007. Application of Giemsa stain for easy detection Trichinella spiralis muscle larvae. Korean Journal of Parasitology, 451, 65–68 doi:10.3347/kjp.2007.45.1.65
Smith, H.J., Messier, S. and Tittiger, F., 1989. Destruction of Trichinella spiralis during the preparation of the “Dry Cured” pork products proscuitto, proscuittini and genoa salami. Canadian Journal of Veterinary Research. 53, 80–83.
Theodoropoulos, G., Kapel, C.M., Webster, P., Saravanos, L., Zaki, J. and Koutsotolis, K., 2000. Infectivity, predilection sites, and freeze tolerance of Trichinella spp. in experimentally infected sheep. Parasitology Research, 86(5), 401–405. doi:10.1007/s004360050684
Acknowledgements
The authors wish to acknowledge Robertina Marín and Concepción Franco (Centro Estatal de Laboratorios Jalisco, Ministry of Health) for technical support to serum samples testing by ELISA. Carlos Pacheco, Silvia Rubalcava and Delia Gonzalez (Departamento de Salud Pública de Ciencias Veterinarias, Universidad de Guadalajara) for technical support. We also thank Raquel Tapia-Romero for critical reading of the manuscript and Daniela González-Ruenes for review of the English used.
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Medina-Lerena, M.S., Ramirez-Álvarez, A., Kühne, M. et al. Influence of different processing procedures on the reproductive capacity of Trichinella spiralis in pork meat. Trop Anim Health Prod 41, 437–442 (2009). https://doi.org/10.1007/s11250-008-9211-9
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DOI: https://doi.org/10.1007/s11250-008-9211-9