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Impaired erythrocyte deformability in transgenic HO-1G143H mutant mice

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Abstract

To investigate the potential effects of variation of HO-1 activity on hemorheology, this study compared the hemorheological properties between transgenic HO-1G143H mutant mice and wild-type (WT) control mice. Fresh blood samples were obtained from mice via the ocular venous sinus. The whole blood viscosity was measured using a cone–plate viscometer. Erythrocyte deformability and aggregation was measured using ektacytometry. The elongation index was significantly reduced in the HO-1G143H mutant mice compared to the WT mice at the shear rates of 600, 800, and 1,000 s−1. The integrated elongation index was decreased in the HO-1G143H mutant mice compared to the WT mice. There was no statistically significant difference between the HO-1G143H mutant mice and the WT mice in terms of whole blood viscosity, aggregation index, amplitude of aggregation, and aggregation half time. The present study demonstrated that a reduction in HO-1 activity results in an impaired erythrocyte deformability. Although the mechanism underlying this effect remains unclear, our study brings to light the participation of HO-1 in the variations of hemorheology.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (No. 31271001) and the National High Technology Research and Development Program of China (No. 2012AA021902).

Conflict of interest

None declared.

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Authors and Affiliations

  1. Institute of Transfusion Medicine, Academy of Military Medical Sciences, No. 27th Taiping Road, HaiDian, Beijing, China

    Gan Chen, Yujing Yin, Bo Wang, Penglong Li, Qingjun Liu, Guoxing You, Jingxiang Zhao, Sha Xia, Lian Zhao & Hong Zhou

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  1. Gan Chen
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  2. Yujing Yin
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  3. Bo Wang
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  4. Penglong Li
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  5. Qingjun Liu
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  7. Jingxiang Zhao
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  8. Sha Xia
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  9. Lian Zhao
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  10. Hong Zhou
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Corresponding authors

Correspondence to Lian Zhao or Hong Zhou.

Additional information

Gan Chen and Yujing Yin have contributed equally to this work.

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Chen, G., Yin, Y., Wang, B. et al. Impaired erythrocyte deformability in transgenic HO-1G143H mutant mice. Transgenic Res 24, 173–178 (2015). https://doi.org/10.1007/s11248-014-9829-5

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  • DOI: https://doi.org/10.1007/s11248-014-9829-5

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