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Usage of putative zebrafish U6 promoters to express shRNA in Nile tilapia and shrimp cell extracts

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Abstract

We conducted in vitro transcription activities of the three zebrafish U6 putative promoters across species in cell extracts prepared from Nile tilapia (Oreochromis niloticus) and shrimps. The transcription efficiency of these putative U6 promoters in Nile tilapia cell extracts was similar to that of zebrafish cell extracts. In addition, all three zebrafish U6 snRNA promoters were able to express the shRNA in cell extracts prepared from two shrimp species, Penaeus monodon and Litopenaeus vannamei. However, the shRNA transcription products in shrimp cell extracts showed weaker signals. These U6 promoters could promote shRNA expression, suggesting that they have the potential for use for vector-based RNAi in Nile tilapia and shrimps. A putative U6 promoter would provide a powerful tool for long-term GKD in these aquaculture-related species.

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References

  • Boonanuntanasarn S, Panyim S, Yoshizaki G (2008) Characterization and organization of the U6 snRNA gene in the zebrafish and usage of their promoters to express short hairpin RNA. Marine Genomics. doi: 10.1016/j.margen.2008.10.001

  • Das G, Henning D, Reddy R (1987) Structure, organization, and transcription of Drosophila U6 small nuclear RNA genes. J Biol Chem 262:1187–1193

    PubMed  CAS  Google Scholar 

  • Das G, Henning D, Wright D, Reddy R (1988) Upstream regulatory elements are necessary and sufficient for transcription of a U6 RNA gene by RNA polymerase III. EMBO J 7:503–512

    PubMed  CAS  Google Scholar 

  • Krol A, Carbon P, Ebel J-P, Appel B (1987) Xenopus tropicalis U6 snRNA genes transcribed by Pol III contain the upstream promoter elements used by Pol II dependent U snRNA genes. Nucleic Acids Res 15:2463–2478

    Article  PubMed  CAS  Google Scholar 

  • Kudo T, Sutou S (2005) Usage of putative chicken U6 promoters for vector-based RNA interference. J Reprod Dev 51:411–417

    Article  PubMed  CAS  Google Scholar 

  • Kunkel GR, Pederson T (1988) Upstream elements required for efficient transcription of a human U6 RNA gene resemble those of U1 and U2 genes even though a different polymerase is used. Genes Dev 2:196–204

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work is supported by a grant from the Thailand Research Funds (MRG 4880201). We thank Prof. Piamsak Menasveta and Assoc. Prof. Somkiat Piyatiratitivorakul (Department of Marine Science, Chulalongkorn University) and Mr. Sunai Plymee (Fisheries Farm, SUT Farm) for providing the marine shrimp larvae and Nile tilapia fry. We also thank Ms. Amolwan Sang-on (Center of Scientific and Technological Equipment) for technical assistance.

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Authors and Affiliations

  1. School of Animal Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang, Nakhon Ratchasima, 30000, Thailand

    Surintorn Boonanuntanasarn

  2. Institute of Molecular Biology and Genetics, Mahidol University, Nakhon Pathom, Thailand

    Sakol Panyim

  3. Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand

    Sakol Panyim

  4. Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato-Ku, Tokyo, 108-8477, Japan

    Goro Yoshizaki

Authors
  1. Surintorn Boonanuntanasarn
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  2. Sakol Panyim
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  3. Goro Yoshizaki
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Correspondence to Surintorn Boonanuntanasarn.

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Boonanuntanasarn, S., Panyim, S. & Yoshizaki, G. Usage of putative zebrafish U6 promoters to express shRNA in Nile tilapia and shrimp cell extracts. Transgenic Res 18, 323–325 (2009). https://doi.org/10.1007/s11248-009-9249-0

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  • DOI: https://doi.org/10.1007/s11248-009-9249-0

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