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Germ line transmission and expression of an RNAi cassette in mice generated by a lentiviral vector system

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Abstract

We have used a lentiviral delivery system (LentiLox3.7) to generate transgenic mice harbouring RNA interference (RNAi) against the hepatocyte nuclear factor 4 gamma (HNF4γ). HNF4γ is a nuclear receptor with unknown function. Our analyses performed on founder (F0) and first generation (F1) mice revealed mosaicism in F0 founders and a low efficiency of transgenesis (6%) in F1 mice. These data, together with the observation of multiple silenced transgenes, do not favour the use of LentiLox3.7 lentivirus for transgenesis. Despite the low efficiency of transgenesis, we achieved a tissue-dependent knockdown of HNF4γ expression in some mice.

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Acknowledgements

We would like to thank Dr. Herbert Spring from the Biomedical Structure Analysis Group (A0600) DKFZ for the help with confocal microscopy and to Dr. David Engblom for critical reading of the manuscript. This work was supported by the “Deutsche Forschungsgemeinschaft” through Collaborative Research Centres SFB 488 and SFB 636, FOR Ot 165/2-2, GRK 791/1.02, and Sachbeihilfe Schu 51/7-2, by the “Fonds der Chemischen Industrie”, the European Union through grant LSHM-CT-2005-018652 (CRESCENDO), the Bundesministerium für Bildung und Forschung (BMBF) through NGFN grants FZK 01GS01117, 01GS0477 and KGCV1/01GS0416, German-Polish Cooperation project 01GZ0310, and project number 0313074C (Systems Biology).

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Authors and Affiliations

  1. Department of Molecular Biology of the Cell I, German Cancer Research Center, Im Neuenheimer Feld 280, 69120, Heidelberg, Germany

    Milen Kirilov, Minqiang Chai, Wolfgang Schmid & Günther Schütz

  2. Transgenic Core Facility, German Cancer Research Center, Im Neuenheimer Feld 280, 69120, Heidelberg, Germany

    Frank van der Hoeven & Ulrich Kloz

Authors
  1. Milen Kirilov
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  2. Minqiang Chai
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  3. Frank van der Hoeven
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  4. Ulrich Kloz
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  5. Wolfgang Schmid
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  6. Günther Schütz
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Correspondence to Günther Schütz.

Additional information

Milen Kirilov and Minqiang Chai contributed equally to this work.

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Kirilov, M., Chai, M., van der Hoeven, F. et al. Germ line transmission and expression of an RNAi cassette in mice generated by a lentiviral vector system. Transgenic Res 16, 783–793 (2007). https://doi.org/10.1007/s11248-007-9119-6

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  • DOI: https://doi.org/10.1007/s11248-007-9119-6

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