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Effects of potato plants expressing a barley cystatin on the predatory bug Podisus maculiventris via herbivorous prey feeding on the plant

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Abstract

The aim of this study was to assess the effects of potato plants expressing a barley cystatin on a potentially cystatin-susceptible natural enemy by predation on susceptible and non-susceptible preys feeding on the plant. We have focussed on the impact of the variant HvCPI-1 C68 → G, in which the only cysteine residue was changed by a glycine, on the growth and digestive physiology of the Colorado potato beetle (CPB), Leptinotarsa decemlineata, and the Egyptian cotton leafworm (ECW), Spodoptera littoralis. Moreover, we have studied the prey-mediated effects of the barley cystatin at the third trophic level, using the predatory spined soldier bug (SSB), Podisus maculiventris, as a model. Feeding trials conducted with CPB larvae reared on transgenic potato plants expressing the C68 → G variant resulted in significantly lower weight gains compared to those fed on non-transformed (NT) plants. On the contrary, larger weight gains were obtained when ECW larvae, that lack digestive cysteine proteases, were reared on transgenic potato expressing the cystatin, as compared to larvae fed on NT plants. No negative effects on survival and growth were observed when SSB nymphs were exposed to HvCPI-1 C68 → G by predation on either CPB or ECW larvae reared on transgenic potato plants expressing the barley cystatin, despite the fact that the inhibitor suppressed in vitro gut proteolysis of the predatory bug. To investigate the physiological background, biochemical analysis were carried out on guts of insects dissected at the end of the feeding assays.

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Acknowledgments

We thank Elisa Viñuela (ETSIA-UPM, Spain) for providing the laboratory colony of S. littoralis. We are also grateful to Pilar Carbonero for a critical reading of the manuscript. This work was supported by “Comunidad de Madrid” (Grants 07M/0050/2002 and 07M/0051/2002). M.M. is a recipient of a Ramon y Cajal contract from the Ministerio de Educación y Ciencia (Spain).

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Authors and Affiliations

  1. Departamento de Biología de Plantas, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain

    Fernando Álvarez-Alfageme, Sara Pascual-Ruiz, Pedro Castañera & Félix Ortego

  2. Laboratorio de Bioquímica y Biología Molecular, Departamento de Biotecnología-UPM, E.T.S. Ingenieros Agrónomos, 28040, Madrid, Spain

    Manuel Martínez & Isabel Diaz

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  1. Fernando Álvarez-Alfageme
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  2. Manuel Martínez
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  3. Sara Pascual-Ruiz
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  4. Pedro Castañera
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  5. Isabel Diaz
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  6. Félix Ortego
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Correspondence to Félix Ortego.

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Fernando Álvarez-Alfageme and Manuel Martínez are contributed equally to this work.

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Álvarez-Alfageme, F., Martínez, M., Pascual-Ruiz, S. et al. Effects of potato plants expressing a barley cystatin on the predatory bug Podisus maculiventris via herbivorous prey feeding on the plant. Transgenic Res 16, 1–13 (2007). https://doi.org/10.1007/s11248-006-9022-6

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  • DOI: https://doi.org/10.1007/s11248-006-9022-6

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