Abstract
Long-term subculture plays an essential role in the large-scale multiplication and production of somatic plantlets. We investigated the effects of long-term subculture on in vitro shoot development and ex vitro rooting associated with changes in the hormones and protein profiles in Cedrela fissilis. The number of subcultures of shoots induced a decrease in the ex vitro rooting response. The reduction in adventitious root (AR) formation was associated with decreases in the contents of indole-3-acetic acid (IAA), abscisic acid (ABA), 12-oxo-phytodienoic acid (OPDA), putrescine (Put), and spermine and increases in jasmonic acid (JA), jasmonoyl-isoleucine, trans-cinnamic acid, and salicylic acid contents in shoots at the fourth subculture compared to the first. The ornithine decarboxylase enzyme preferentially functions in the Put biosynthesis pathway, and it was related to the highest AR formation in shoots at the first subculture. Down-accumulation of the auxin-binding protein ABP19a in shoots from the fourth subculture compared to the first subculture was related to a decrease in both the IAA content and AR formation. In addition, down-accumulation of glucose-6-phosphate isomerase; glutamine synthetase leaf isozyme, chloroplastic; 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase; l-ascorbate peroxidase, cytosolic; monodehydroascorbate reductase; and 2-Cys peroxiredoxin BAS1-like, chloroplastic and up-accumulation of caffeoyl-CoA O-methyltransferase 1 and isoforms of peroxidase 4 proteins in shoots from the fourth relative to the first subculture were associated with a reduction in AR formation. These results showed that the understanding of hormonal and molecular mechanisms related to the potential of AR formation in shoots under successive subcultures is relevant to improving large-scale plantlet production in C. fissilis.
Key message
Long-term subculture affects the competence to ex vitro rooting of C. fissilis shoots by changes in endogenous hormones and protein profiles.
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Data availability
The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD023173. The list of all identified proteins is available in the supplementary material.
Code availability
PXD023173.
Abbreviations
- ABA:
-
Abscisic acid
- ADC:
-
Arginine decarboxylase
- ARs:
-
Adventitious roots
- BA:
-
6-Benzyladenine
- DAPs:
-
Differentially accumulated proteins
- DM:
-
Dry matter
- DTT:
-
Dithiothreitol
- EDTA:
-
Ethylenediaminetetraacetic acid
- FM:
-
Fresh matter
- HPLC:
-
High-performance liquid chromatography
- IAA:
-
Indole-3-acetic acid
- JA:
-
Jasmonic acid
- JA-Ile:
-
Jasmonoyl-isoleucine
- MS:
-
Murashige and Skoog culture medium
- ODC:
-
Ornithine decarboxylase
- PAs:
-
Polyamines
- Put:
-
Putrescine
- SA:
-
Salicylic acid
- Spd:
-
Spermidine
- Spm:
-
Spermine
- TCA:
-
Trichloroacetic acid
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Acknowledgements
Funding for this work was provided by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (444453/2014-8; 309,303/2019-2) and the Fundação Carlos Chagas Filho de Amparo à Pesquisa no Estado do Rio de Janeiro (FAPERJ) (E26/202.969/2016; E26/202.533/2019). This study was also funded by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001. The plant hormone measurements were performed at SCIC from UJI and were supported by Grant UJI-B2019-11. TRO is thankful for the scholarship funding provided by FAPERJ and CAPES – Finance Code 001. VPMA acknowledges postdoctoral scholarship (E-26/202.295/2017) funding from FAPERJ. KRS acknowledge the scholarship funding from FAPERJ.
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CSC and TRO conceived the study, designed the experiments, and wrote the manuscript. TRO was responsible for the in vitro culture experiments, PA analyses and statistical analyses. TRO, KRS and VS were responsible for the proteomic analyses. TRO, DB and AGC were responsible for the plant hormone analyses at SCIC from UJI – Spain. TRO, VPMA, LFO and EISF were responsible for the ADC and ODC enzyme analyses. All the authors read and approved the final manuscript.
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dos Reis de Oliveira, T., Balfagón, D., Sousa, K.R. et al. Long-term subculture affects rooting competence via changes in the hormones and protein profiles in Cedrela fissilis Vell. (Meliaceae) shoots. Plant Cell Tiss Organ Cult 148, 137–153 (2022). https://doi.org/10.1007/s11240-021-02172-6
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DOI: https://doi.org/10.1007/s11240-021-02172-6