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Assessments of rooting, vegetative growth, bulb production, genetic integrity and biochemical compounds in cryopreserved plants of shallot

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Abstract

Shallot (Allium cepa var. aggregatum), a small bulb onion, is widely grown in the world. We previously reported a droplet-vitrification for cryopreservation of in vitro-grown shoot tips of shallot genotype ‘10603’. The present study further evaluated rooting, vegetative growth, bulb production and contents of biochemical compounds, as well as genetic stability in cryo-derived plants. The results showed no significant differences in rooting, vegetative growth, bulb production and contents of soluble sugars and flavonols between the cryo- and in vitro-derived plants. Analyses of ISSR and AFLP markers did not detect any polymorphic bands in the cryo-derived plants. These results indicate rooting and vegetative growth ability, biochemical compounds and genetic stability were maintained in cryo-derived plants. The present study provides experimental evidences that support the use of cryopreservation method for long-term preservation of genetic resources of shallots and other Allium species.

Key message

Rooting, vegetative growth, bulb production, genetic stability and biochemical compounds were maintained in cryopreserved plants of shallot. Our results support use of cryopreservation for long-term preservation of shallot germplasm.

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Abbreviations

AFLP:

Amplified fragment length polymorphism

6-BA:

6-Benzylaminopurine

ISSR:

Inter-simple sequence repeat

LN:

Liquid nitrogen

LNV:

Liquid nitrogen vapor

MS:

Murashige and Skoog (1962)

NAA:

1-Naphthylacetic acid

SCM:

Stock culture medium

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Acknowledgements

We acknowledge financial supports from the Research Council of Norway (Project No. 255032/E50), NIBIO, Sagaplant, Gartnerhallen, Gartnerforbundet, and Landbruksdirektoratet/the Norwegian Genetic Resource Centre (Project No. 18/4272).

Author information

Authors and Affiliations

  1. Division of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research, Ås, Norway

    Min-Rui Wang, Zhibo Hamborg, Abdelhameed Elameen, Dag-Ragnar Blystad & Sissel Haugslien

  2. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Min-Rui Wang & Qiao-Chun Wang

  3. PlantChem AS, Eikenveien 334, 4596, Eiken, Norway

    Rune Slimestad

  4. Department of Plant Sciences, Faculty of Biosciences, Norwegian University of Life Sciences, Ås, Norway

    Gry Skjeseth

Authors
  1. Min-Rui Wang
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  2. Zhibo Hamborg
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  3. Rune Slimestad
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  4. Abdelhameed Elameen
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  5. Dag-Ragnar Blystad
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  6. Sissel Haugslien
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  7. Gry Skjeseth
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  8. Qiao-Chun Wang
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Contributions

MRW: performance of experiments of cryopreservation, rooting and vegetative growth, collection and analysis of data, and preparation of manuscript; ZZ: performance of experiments of genetic stability and biochemical compounds, collection and analysis of data and preparation of manuscript; RS: analysis of biochemical compounds; AE: performance of assessments of genetic stability, valuable discussion and revision of manuscript; DRB: chief scientist of the project, valuable discussion and providing financial supports; SH: maintenance of in vitro cultures, and assistance to experiments of cryopreservation and evaluations of in vitro rooting and vegetatitive growth; GS: maintenance of greenhouse-grown plants and evaluations of vegetative growth in greenhouse-grown plants; QCW: experimental design, analysis of data, and revision and editing of manuscript.

Corresponding authors

Correspondence to Zhibo Hamborg, Abdelhameed Elameen or Dag-Ragnar Blystad.

Additional information

Communicated by M. Angeles Revilla.

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Wang, MR., Hamborg, Z., Slimestad, R. et al. Assessments of rooting, vegetative growth, bulb production, genetic integrity and biochemical compounds in cryopreserved plants of shallot. Plant Cell Tiss Organ Cult 144, 123–131 (2021). https://doi.org/10.1007/s11240-020-01820-7

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  • DOI: https://doi.org/10.1007/s11240-020-01820-7

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