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Metabolomics reveals ionones upregulation in MeJA elicited Cinnamomum camphora (camphor tree) cell culture

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Abstract

Cell suspension culture offers an approach for elucidating secondary metabolites biosynthetic pathways and its regulatory mechanism. In this work, Cinnamomum camphora (camphor tree) a plant enriched in essential oil components and employed in traditional medicine for the treatment of several conditions was subjected to callus induction. Callus growth was optimized in terms of medium type and concentration of plant growth regulators, revealing that Murashige and Skoog (MS) medium containing 2.0 mg L−1 naphthalene acetic acid plus 1.0 mg L−1 6-benzylaminopurine yielded the best combination for callus growth. Moreover, an MS-based metabolomics approach was used to compare volatile profile of intact leaf versus callus. Few reports in planta have addressed differences in volatiles composition between cell cultures (callus) and its original explants using such large scale analytical approaches. Headspace solid phase microextraction coupled to gas chromatography mass spectrometry was utilized to profile C. camphora leaf volatiles with a total of 47 volatiles including monoterpenoids viz., cineole and β-phellandrene as major constituents. In contrast, callus volatile profile showed qualitative and quantitative differences from that of leaf tissue and with its aroma being mostly dominated by aldehydes viz., benzaldehyde. Methyl jasmonate (MeJA) phytohormone elicitation effect was further applied with the aim of enhancing flavours volatiles production. Multivariate data analyses revealed that MeJA led to the upregulation of ionones production first time to be reported in C. comphora. This study extends our knowledge regarding ionones formation and to extend MeJA as a potential inducer of such flavour volatile class in planta.

Key message

Cinnamomum camphora was subjected to callus induction. A metabolomics approach was used to compare callus profile to that of leaf. MeJA elicitation upregulated ionones class first time to be reported.

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Authors and Affiliations

  1. Timber Trees Research Department, Horticulture Research Institute, Agriculture Research Center, Cairo, Egypt

    Essam M. Abd El-Kader

  2. Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo, 11751, Egypt

    Ahmed Serag

  3. Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt

    Mohamed S. Aref & Emad E. A. Ewais

  4. Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt

    Mohamed A. Farag

  5. Chemistry Department, School of Sciences & Engineering, The American University in Cairo, New Cairo, Egypt

    Mohamed A. Farag

Authors
  1. Essam M. Abd El-Kader
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  2. Ahmed Serag
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  3. Mohamed S. Aref
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  4. Emad E. A. Ewais
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  5. Mohamed A. Farag
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Contributions

EA, MA and EE performed the callus experiments and quantified the data. AS analysed the results and wrote the paper. MAF performed volatiles analysis, data interpretation and edited the manuscript.

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Correspondence to Mohamed A. Farag.

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Communicated by Mohammad Faisal.

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Abd El-Kader, E.M., Serag, A., Aref, M.S. et al. Metabolomics reveals ionones upregulation in MeJA elicited Cinnamomum camphora (camphor tree) cell culture. Plant Cell Tiss Organ Cult 137, 309–318 (2019). https://doi.org/10.1007/s11240-019-01572-z

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  • DOI: https://doi.org/10.1007/s11240-019-01572-z

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