Skip to main content

Advertisement

SpringerLink
Log in

In vitro multiplication of wild relatives in genus Beta conserves the invaluable threatened germplasms

  • Research Note
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

Conservation of threatened plant genetic resources is vital for food security and biodiversity maintenance. Wild species of genus Beta are invaluable germplasm which could be utilized for beet breeding programs. In this study, in vitro multiplication and genotypic responses of different species in the section Beta and Corollinae were compared in presence of plant growth regulators. Following the surface sterilization, seeds were cultivated in vitro. Cotyledonary node explants were excised from the germinated young seedlings and treated with BAP, GA3 and TDZ. The obtained results demonstrated a significant difference between the genotypes in terms of in vitro shoot proliferation efficiency when subjected to BAP and GA3. Among the applied growth regulators, effect of BAP on number of multiplied shoots was the highest. Moreover, the genotypic variation in rooting was also evident when the shoots were subjected to IAA and IBA. This study is the first report of in vitro multiplication of wild species in genus Beta that could be exploited in future germplasm maintenance, gene transformation and breeding studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Abbreviations

BAP:

6-Benzylaminopurine

GA3:

Gibberellic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog Basal Medium

TDZ:

Thidiazuron

References

  • Asher MJC, Luterbacher MC, Frese L (2001) Wild beta species as a source of resistance to sugar beet pests and diseases. Proc IIRB 64:141–152

    Google Scholar 

  • Astly D (1992) Reservation of genetic diversity and accession integrity. Field Crops Res 29:205–224

    Article  Google Scholar 

  • Biaggi MD, Biancardi E (2016) Breeding methods. In: Biancardi E, Tamada T (eds) Rhizomania Springer, Cham, pp 233–248

    Chapter  Google Scholar 

  • Biancardi E, Panella LW, Lewellen RT (2012) Beta maritima: the origin of beets. Springer, New York

    Book  Google Scholar 

  • Capistrano-Gossmann GG, Ries D, Holtgräwe D (2017) Crop wild relative populations of Beta vulgaris allow direct mapping of agronomically important genes. Nat Commun 8:15708. https://doi.org/10.1038/ncomms15708

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Coons GH (1975) Interspecific hybrids between Beta vulgaris L. and the wild species of Beta. J Am Soc Sugar Beet Technol 18:281–306

    Article  Google Scholar 

  • Ford-Lloyd BV (2005) Sources of genetic variation, Genus Beta. In: Biancardi E, Campbell LG, Skaracis GN, De Biaggi M (eds) Genetics and breeding of sugar beet, Science Publishers Inc, Enfield, pp 25–33

    Google Scholar 

  • Ford-Lloyd BV, Schmidt M, Armstrong SJ et al (2011) Crop wild relatives undervalued, underutilized and under threat? BioScience 61(7):559–565. https://doi.org/10.1525/bio.2011.61.7.10

    Article  Google Scholar 

  • Golden AM (1959) Susceptibility of several Beta species to the sugar beet nematode (Heterodera schachtbii) and root-knot nematodes (Meloidogyne spp.). J Am Soc Sugar Beet Technol 10:444–447

    Article  Google Scholar 

  • Gürel S, Gürel E, Kaya Z (2001) Callus development and indirect shoot regeneration from seedling explants of sugar beet (Beta vulgaris L.) cultured in vitro. Turk J Bot 25:25–33

    Google Scholar 

  • Gürel S, Topal E, Gürel E (2003) The effect of pretreating seedlings with TDZ on direct shoot regeneration from petiole explants of sugar beet (Beta vulgaris L.). Asia Pac J Mol Biol Biotechnol 11(1):57–62

    Google Scholar 

  • Jacq B, Tetu T, Sangwan RS, Laat AD, Sangwan-Norreel BS (1992) Plant regeneration from sugar beet (Beta vulgaris L.) hypocotyls cultured in vitro and flow cytometric nuclear DNA analysis of regenerants. Plant Cell Rep 11: 329–333

    Article  PubMed  CAS  Google Scholar 

  • Kadereit G, Hohmann S, Kadereit JW (2006) A synopsis of Chenopodiaceae subfam. Betoideae and notes on the taxonomy of Beta. Willdenowia 36:9–19

    Article  Google Scholar 

  • Kagami H, Taguchi K, Arakawa T, Kuroda Y, Tamagake H, Hereditas TK (2016) Efficient callus formation and plant regeneration are heritable characters in sugar beet (Beta vulgaris L.). Hereditas 153:12. https://doi.org/10.1186/s41065-016-0015-z

    Article  PubMed  PubMed Central  Google Scholar 

  • Kulshrestha S, Coutts RHA (1997) Direct somatic embryogenesis and plant regeneration from mature sugarbeet (Beta vulgaris L.) zygotic cotyledons. Plant Growth Regul 22(2):87–92

    Article  Google Scholar 

  • Maxted N, Kell S (2009) Establishment of a global network for the in situ conservation of crop wild relatives: status and needs. FAO Consultancy Report. http://www.fao.org/docrep/013/i1500e/i1500e18a.pdf Accessed 29 Sep 2017

  • Meilleur BA, Hodgkin T (2004) In situ conservation of crop wild relatives: status and Trends. Biodivers Conserv 13:663–684

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Özgür OM (1992) Beta L. of Turkey. The general directorate of the Turkish sugar corp, Ankara**

  • Panella L, Lewellen RT (2007) Broadening the genetic base of sugarbeet: introgression from wild relatives. Euphytica 154:383–400

    Article  CAS  Google Scholar 

  • Pazuki A, Aflaki F, Gürel S, Ergul A, Gürel E (2018) Production of doubled haploids in sugar beet (Beta vulgaris): an efficient method by a multivariate experiment. Plant Cell Tiss Organ Cult 132(1):85–97. https://doi.org/10.1007/s11240-017-1313-5

    Article  CAS  Google Scholar 

  • Rajpurohit D, Jhang T (2015) In situ and ex situ conservation of plant genetic resources and traditional knowledge. In: Salgotra R, Gupta B (eds) Plant genetic resources and traditional knowledge for food security. Springer, Singapore, pp 137–162

    Chapter  Google Scholar 

  • Reamon-Büttner SM, Wricke G (1993) Evidence of tetrasomic inheritance in Beta corolliflora. J Sugar Beet Res 30:321–327

    Article  Google Scholar 

  • Ren H, Zhang Q, Lu H et al (2012) Wild plant species with extremely small populations require conservation and reintroduction in China. Ambio 41(8):913–917. https://doi.org/10.1007/s13280-012-0284-3

    Article  PubMed  PubMed Central  Google Scholar 

  • Saunders JW, Doley WP (1986) One step shoot regeneration from callus of whole plant leaf explants of sugarbeet lines and a somaclonal variant for in vitro behavior. J Plant Physiol 124(5):473–479

    Article  CAS  Google Scholar 

  • Scholten OE, De Bock TS, Klein-Lankhorst RM, Lange W (1999) Inheritance of resistance to beet necrotic yellow vein virus in Beta vulgaris conferred by a second gene for resistance. Theor Appl Genet 99:740–746

    Article  PubMed  CAS  Google Scholar 

  • Seiler GJ, Frese L, Burenin V (1996) Collection of wild beta section Corollinae in the Soviet Union. J Sugar Beet Res 33(1–4):31–43

    Article  Google Scholar 

  • Tamada T, Baba T (1973) Beet necrotic yellow vein virus from rhizomaniaaffected sugar beet in Japan. Ann Phytopathol Soc Jpn 39:325–332

    Article  Google Scholar 

  • Yu MH (1989) Callus induction and differentiation from leaf explants of different species of the genus Beta. Crop Sci 29:205–209

    Article  Google Scholar 

Download references

Acknowledgements

The financial support from the Scientific and Technological Research Council of Turkey (TUBITAK), Project No: TOVAG-113O096, is deeply appreciated. Also, the authors are thankful to the Aegean Agricultural Research Institute, Izmir, Turkey, for kindly providing the seeds required for the study.

Author information

Authors and Affiliations

  1. Biotechnology Institute, Ankara University, 06110, Ankara, Turkey

    A. Ergül, S. D. Khabbazi, M. Ç. Oğuz & C. Y. Özmen

  2. Department of Plant Breeding, Sugar Institute, Etimesgut, 06930, Ankara, Turkey

    S. Gürel

  3. Department of Biology, Abant Izzet Baysal University, 14030, Bolu, Turkey

    E. Gürel

Authors
  1. A. Ergül
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. D. Khabbazi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Ç. Oğuz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Y. Özmen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. Gürel
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. E. Gürel
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

AE designed and outlined the research. SDK contributed to data analysis, interpreted the data and wrote the manuscript. MÇO analyzed the data. MÇO and CYÖ performed the experiment. SG and EG criticized the data interpretation and edited the manuscript.

Corresponding author

Correspondence to A. Ergül.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Communicated by Sergio J. Ochatt.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ergül, A., Khabbazi, S.D., Oğuz, M.Ç. et al. In vitro multiplication of wild relatives in genus Beta conserves the invaluable threatened germplasms. Plant Cell Tiss Organ Cult 134, 169–175 (2018). https://doi.org/10.1007/s11240-018-1410-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-018-1410-0

Keywords

Search

Navigation