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Proteomic analysis of Mammillaria gracilis Pfeiff. in vitro-grown cultures exposed to iso-osmotic NaCl and mannitol

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Abstract

Salt and drought stress are important abiotic factors that negatively affect plant growth and productivity. Defense mechanisms, which plants have developed to cope with stress, are followed by alterations in a genome expression profile that in turn result in qualitative and quantitative change of the proteome. Although proteomic-based approach for studies of plant responses to salinity and drought has already been successfully employed in several plants, for cactus species such analyses have not been done so far. Therefore, in this study we have performed proteomic analysis of Mammillaria gracilis Pfeiff. in vitro-grown cultures, callus and tumor, exposed to iso-osmotic NaCl and mannitol. Obtained results differed among analyzed tissues. The higher number of differentially expressed proteins after either salt or mannitol treatment was revealed in tumor compared to callus. According to classification to different functional categories, majority of the identified callus responsive proteins belongs to protein synthesis and processing category, while the highest number of identified tumor proteins belongs to category of metabolism, which suggest that the mechanisms that mediate responses to salt- and mannitol-induced stress in cactus callus and tumor are dependent on tissue type. Down-regulation of proteins involved in cell protection suggests the inability of tumor to activate protective processes against salinity and osmotic stress.

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Acknowledgments

The financial support of this work was provided by The Ministry of Science, Education and Sports of the Republic of Croatia (119-1191196-1200) as well as by the University of Zagreb.

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  1. Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000, Zagreb, Croatia

    Tea Rogić & Biljana Balen

  2. Ruđer Bošković Institute, POB 1016, 10 000, Zagreb, Croatia

    Anita Horvatić & Mario Cindrić

  3. Division of Botany, Department of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10 000, Zagreb, Croatia

    Mirta Tkalec

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  1. Tea Rogić
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Rogić, T., Horvatić, A., Tkalec, M. et al. Proteomic analysis of Mammillaria gracilis Pfeiff. in vitro-grown cultures exposed to iso-osmotic NaCl and mannitol. Plant Cell Tiss Organ Cult 122, 127–146 (2015). https://doi.org/10.1007/s11240-015-0756-9

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  • DOI: https://doi.org/10.1007/s11240-015-0756-9

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