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Overexpression of SaRBP1 enhances tolerance of Arabidopsis to salt stress

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Abstract

Abiotic stresses are the major concern in recent years as their effect on world food production is constantly increasing. We have obtained salt tolerant Arabidopsis lines overexpressing SaRBP1 (Suaeda asparagoides RNA binding protein 1) of a Korean halophyte, S. asparagoides. Homozygous T3 Arabidopsis transgenic lines were developed and used for salt stress tolerance studies. The transgenic seedlings displayed tolerance to salt and mannitol compared to the wild type (WT) seedlings. Transgenic lines produced longer primary roots, more fresh weight, and higher number of lateral roots than WT. In planta stress tolerance assay results showed that the survival rates of transgenic plants were significantly higher than WT plants. Transgenic lines showed delayed germination under 200 mM NaCl stress. In addition, the transgenics showed higher water retention ability than WT. Subcellular localization results revealed that SaRBP1 was targeted to the cytoplasm. Northwestern blot analysis results confirmed the RNA binding property of SaRBP1. Quantitative Real-Time Polymerase Chain Reaction results revealed that many stress marker genes were upregulated by SaRBP1 overexpression. Thus, our data demonstrate that SaRBP1 overexpression lines are tolerant to salt stress. Hence, this is the first report for the functional characterization of SaRBP1, a novel RBP gene isolated from S. asparagoides cDNA library.

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Abbreviations

ABA:

Abscisic acid

GFP:

Green fluorescent protein

WT:

Wild type

SaRBP1:

Suaeda asparagoides RNA binding protein 1

GRP:

Glycine-rich RNA binding protein

EGFP:

Enhanced green fluorescent protein

GST:

Glutathione S-transferase

SDS-PAGE:

Sodium dodecyl sulfate-poly acrylamide gel electrophoresis

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Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (KRF) funded by the Ministry of Education, Science and Technology (KRF-2012-001205 and -2012-001273), and the support of Cooperative Research Program for Agriculture Science and Technology Development (Project title: Isolation and functional analyses of novel genes involved in resistance to abiotic stress for improved soybean productivity, Project No. PJ007970), Rural Development Administration, Republic of Korea.

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  1. Department of Genetic Engineering, Dong-A University, Busan, 604714, Korea

    Selvam Ayarpadikannan, Eun Sook Chung, Hyun Ah So, Kyoung Mi Kim, Kenneth Ryan Schraufnagle & Jai Heon Lee

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  1. Selvam Ayarpadikannan
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  2. Eun Sook Chung
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  3. Hyun Ah So
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Correspondence to Jai Heon Lee.

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Ayarpadikannan, S., Chung, E.S., So, H.A. et al. Overexpression of SaRBP1 enhances tolerance of Arabidopsis to salt stress. Plant Cell Tiss Organ Cult 118, 327–338 (2014). https://doi.org/10.1007/s11240-014-0485-5

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