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Dependence of UV-B-induced camptothecin production on nitrate reductase-mediated nitric oxide signaling in Camptotheca acuminata suspension cell cultures

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Abstract

UV-B irradiation induced production of secondary metabolites in plant cells. However, the mechanisms of UV-B-induced secondary metabolite production remained largely unknown. Here we report that UV-B treatment stimulated nitric oxide (NO) generation and camptothecin (CPT) production in Camptotheca acuminata cells. To investigate the origin of the UV-B-triggered NO and the role of NO in UV-B-induced CPT production, we assayed the responses of nitrate reductase (NR) and NO synthase (NOS) activities of the cells to UV-B exposure and examined the effects of NR and NOS inhibitors on CPT production in UV-B-treated cells. The data showed that UV-B irradiation enhanced NR activities in the cells. Pretreatment with NR inhibitors tungstate and okadaic acid not only suppressed the UV-B-triggered NR activities but also inhibited the UV-B-induced NO generation and CPT production in the cells. In contrast, UV-B irradiation had no effects on NOS activity of the cells and treatment of NOS inhibitor did not suppress UV-B-induced CAT production. Together, the results demonstrated that NR activity was essential for UV-B-triggered NO generation and that NR-mediated NO signaling was involved in UV-B-induced CPT production in C. acuminata cells.

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Acknowledgments

We gratefully acknowledge Ms Chen Xiaofang for the help of cell culture. This work was financially supported in part by the Natural Science Foundation of China (No. 81072998 and 8137390) and the Natural Science Foundation of Zhejiang Province (No. R2080328).

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Authors and Affiliations

  1. Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, 310035, China

    Jiazhao Ruan, Jinjie Zhang, Mengya Li, Yun Zhu, Lina Sun, Haihong Jin, Hu Su & Maojun Xu

  2. Department of Food Science and Engineering, Ningbo University, Ningbo, 315211, China

    Jinjie Zhang

  3. College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, 330012, China

    Hu Su

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  1. Jiazhao Ruan
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  2. Jinjie Zhang
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Correspondence to Hu Su or Maojun Xu.

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Ruan, J., Zhang, J., Li, M. et al. Dependence of UV-B-induced camptothecin production on nitrate reductase-mediated nitric oxide signaling in Camptotheca acuminata suspension cell cultures. Plant Cell Tiss Organ Cult 118, 269–278 (2014). https://doi.org/10.1007/s11240-014-0479-3

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