Skip to main content
SpringerLink
Log in

Overexpression of ZmAFB2, the maize homologue of AFB2 gene, enhances salt tolerance in transgenic tobacco

  • Original Paper
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

The TIR1/AFB F-box proteins act as auxin receptors and play an important role in auxin mediated plant development. In the present study, a homologue of AFB2 gene, ZmAFB2, has been cloned from maize (Zea mays L.). Expression of ZmAFB2 is induced by NaCl and salicylic acid treatment and reduced by indole-3-acetic acid (IAA) treatment. To identify the function of the ZmAFB2 gene under stress conditions, a binary vector containing ZmAFB2 driven by CaMV 35S promoter was constructed and transformed into tobacco (Nicotiana tabacum) by Agrobacterium-mediated transformation. The transgenic tobacco lines overexpressing ZmAFB2 exhibit increased tolerance to salt treatment. Furthermore, overexpression of ZmAFB2 enhances the transcription levels of stress responsive genes Ascorbate peroxidase and Catalase. These results indicate that ZmAFB2 is involved in abiotic stress response and overexpression of ZmAFB2 enhances salt tolerance in transgenic tobacco.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281–297

    Article  PubMed  CAS  Google Scholar 

  • Bostock RM (2005) Signal crosstalk and induced resistance: straddling the line between cost and benefit. Annu Rev Phytopathol 43:545–580

    Article  PubMed  CAS  Google Scholar 

  • Calderon-Villalobos LI, Tan X, Zheng N, Estelle M (2010) Auxin perception-structural insights. Cold Spring Harb Perspect Biol 2:a005546

    Article  PubMed  Google Scholar 

  • Cecchetti V, Altamura MM, Falasca G, Costantino P, Cardarelli M (2008) Auxin regulates Arabidopsis anther dehiscence, pollen maturation, and filament elongation. Plant Cell 20(7):1760–1774

    Article  PubMed  CAS  Google Scholar 

  • Chen H, Li Z, Xiong L (2012) A plant microRNA regulates the adaptation of roots to drought stress. FEBS Lett 586:1742–1747

    Article  PubMed  CAS  Google Scholar 

  • Dharmasiri N, Estelle M (2004) Auxin signaling and regulated protein degradation. Trends Plant Sci 9:302–308

    Article  PubMed  CAS  Google Scholar 

  • Dharmasiri N, Dharmasiri S et al (2005a) Plant development is regulated by a family of auxin receptor F-box proteins. Dev Cell 9(1):109–119

    Article  PubMed  CAS  Google Scholar 

  • Dharmasiri N, Dharmasiri S, Estelle M (2005b) The F-box protein TIR1 is an auxin receptor. Nature 435:441–445

    Article  PubMed  CAS  Google Scholar 

  • Dowd C, Wilson IW, McFadden H (2004) Gene expression profile changes in cotton root and hypocotyl tissues in response to infection with Fusarium oxysporum f. sp. Vasinfectum. Mol Plant Microbe Interact 17(6):654–667

    Article  PubMed  CAS  Google Scholar 

  • Faize M, Faize L, Burgos L (2010) Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociation. BMC Biotechnol 10:53. doi:10.1186/1472-6750-10-53

    Article  PubMed  Google Scholar 

  • Feng XM, You CX, Qiao Yu, Mao K, Hao YJ (2010) Ectopic overexpression of Arabidopsis AtmiR393a gene changes auxin sensitivity and enhances salt resistance in tobacco. Acta Physiol Plant 32:997–1003

    Article  CAS  Google Scholar 

  • Friml J (2003) Auxin transport - shaping the plant. Curr Opin Plant Biol 6(1):7–12

    Article  PubMed  CAS  Google Scholar 

  • Gao P, Bai X, Yang L, Lv DK, Pan X, Li Y, Cai H, Ji W, Chen Q, Zhu YM (2011) osa-MIR393: a salinity- and alkaline stress-related microRNA gene. Mol Biol Rep 38:237–242

    Article  PubMed  CAS  Google Scholar 

  • Geraint P, Mark E (2006) Auxin receptors: a new role for F-box proteins. Curr Opin Cell Biol 18:152–156

    Article  Google Scholar 

  • Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930

    Article  PubMed  CAS  Google Scholar 

  • Goodman CD, Casati P, Walbot V (2004) A multidrug resistance-associated protein involved in anthocyanin transport in Zea mays. Plant Cell 16:1812–1826

    Article  PubMed  CAS  Google Scholar 

  • Gray WM, Kepinski S, Rouse D, Leyser O, Estelle M (2001) Auxin regulates SCF(TIR1)-dependent degradation of AUX/IAA proteins. Nature 414:271–276

    Article  PubMed  CAS  Google Scholar 

  • Haglund K, Dikic I (2005) Ubiquitylation and cell signaling. EMBO J 24:3353–3359

    Article  PubMed  CAS  Google Scholar 

  • He Y, Liu Y, Cao W, Huai M, Xu B, Huang B (2005) Effects of salicylic acid on heat tolerance associated with antioxidant metabolism in Kentuky Bluegrass. Crop Sci 45:988–995

    Article  CAS  Google Scholar 

  • Huang WL, Lee CH, Chen YR (2012) Levels of endogenous abscisic acid and indole-3-acetic acid influence shoot organogenesis in callus cultures of rice subjected to osmotic stress. Plant Cell Tiss Organ Cult 108:257–263

    Article  CAS  Google Scholar 

  • Iglesias MJ, Terrile MC, Bartoli CG, D’Ippólito S, Casalongué CA (2010) Auxin signaling participates in the adaptative response against oxidative stress and salinity by interacting with redox metabolism in Arabidopsis. Plant Mol Biol 74(3):215–222

    Article  PubMed  CAS  Google Scholar 

  • Iglesias MJ, Terrile MC, Casalongué CA (2011) Auxin and salicylic acid signalings counteract the regulation of adaptive responses to stress. Plant Signal Behav 6(3):452–454

    Article  PubMed  CAS  Google Scholar 

  • Jain M, Khurana JP (2009) Transcript profiling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice. FEBS J 276:3148–3162

    Article  PubMed  CAS  Google Scholar 

  • Jain S, Kumar D, Jain M, Chaudhary P, Deswal R, Sarin NB (2012) Ectopic overexpression of a salt stress-induced pathogenesis-related class 10protein (PR10) gene from peanut (Arachis hypogaea L.) affords broad spectrum abiotic stress tolerance in transgenic tobacco. Plant Cell Tiss Organ Cult 109:19–31

    Article  CAS  Google Scholar 

  • Jones-Rhoades MW, Bartel DP (2004) Computational identification of plant microRNAs and their targets, including a stress induced miRNA. Mol Cell 14(6):787–799

    Article  PubMed  CAS  Google Scholar 

  • Kepinski S, Leyser O (2005) The Arabidopsis F-box protein TIR1 is an auxin receptor. Nature 435:446–451

    Article  PubMed  CAS  Google Scholar 

  • Kim J, Harter K, Theologis A (1997) Protein–protein interactions among the Aux/IAA proteins. PNAS 22:11786–11791

    Article  Google Scholar 

  • Koornneef A, Pieterse CMJ (2008) Cross talk in defense signaling. Plant Physiol 146:839–844

    Article  PubMed  CAS  Google Scholar 

  • Letunic I, Doerks T, Bork P (2009) SMART 6: recent updates and new developments. Nucl Acids Res 37:229–232

    Article  Google Scholar 

  • Navarro L, Dunoyer P, Jay F, Arnold B, Dharmasiri N, Estelle M, Voinnet O, Jones JDG (2006) A plant miRNA contributes to antibacterial resistance by repressing auxin signaling. Science 312:436–439

    Article  PubMed  CAS  Google Scholar 

  • O’Donnell PJ, Schmelz EA, Moussatche P (2003) Susceptible to intolerance—a range of hormonal actions in a susceptible Arabidopsis pathogen response. Plant J 33(2):245–257

    Article  PubMed  Google Scholar 

  • Park JE, Park JY, Kim YS (2007) GH3-mediated auxin homeostasis links growth regulation with stress adaptation response in Arabidopsis. J Biol Chem 282:10036–10046

    Article  PubMed  CAS  Google Scholar 

  • Parry G, Calderon-Villalobos LI, Prigge M, Peret B, Dharmasiri S, Itoh H, Lechner E, Gray WM, Bennett M, Estelle M (2009) Complex regulation of the TIR1/AFB family of auxin receptors. Proc Natl Acad Sci 706:22540–22545

    Article  Google Scholar 

  • Raskin I (1992) Role of salicylic-acid in plants. Annu Rev Plant Biol 43:439–463

    Article  CAS  Google Scholar 

  • Ren ZX, Li ZG, Miao Q, Yang YW, Deng W, Hao YW (2011) The auxin receptor homologue in Solanum lycopersicum stimulates tomato fruit set and leaf morphogenesis. J Exp Bot 62(8):2815–2826

    Article  PubMed  CAS  Google Scholar 

  • Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nat Protoc 3(6):1101–1108

    Article  PubMed  CAS  Google Scholar 

  • Shen CJ, Bai YH, Wang SK, Zhang SN, Wu YR, Chen M, Jiang DA, Qi YH (2010) Expression profile of PIN, AUX/LAX and PGP auxin transporter gene families in Sorghum bicolor under phytohormone and abiotic stress. FEBS J 277:2954–2969

    Article  PubMed  CAS  Google Scholar 

  • Singh B, Usha K (2003) Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regul 39:137–141

    Article  CAS  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  • Tan X, Calderon-Villalobos LI, Sharon M, Zheng C, Robinson CV, Estelle M, Zheng N (2007) Mechanism of auxin perception by the TIR1 ubiquitin ligase. Nature 446:640–645

    Article  PubMed  CAS  Google Scholar 

  • Tasgin E, Atici O, Nalbantoghu B (2003) Effect of salicylic acid and cold on freezing tolerance in wheat leaves. Plant Growth Regul 41:231–236

    Article  CAS  Google Scholar 

  • Tian Y, Zhang CH, Yang H, Lu XY, Fang J, Li PW, Qing XG (2011) Molecular cloning and characterization of a TRANSPORT INHIBITOR RESPONSE 1 (TIR1) from Nicotiana tabacum. Russ J Plant Physiol 58(1):149–156

    Article  CAS  Google Scholar 

  • Tiwari SB, Wang XJ, Hagen G, Guilfoyle TJ (2001) Aux/IAA proteins are active repressors and their stability and activity are modulated by auxin. Plant Cell 13:2809–2822

    PubMed  CAS  Google Scholar 

  • Tiwari SB, Hagen G, Guilfoyle TJ (2004) Aux/IAA proteins contain a potent transcriptional repression domain. Plant Cell 16:533–543

    Article  PubMed  CAS  Google Scholar 

  • Topping JF (1998) Tobacco transformation. Methods Mol Biol 81:365–372. doi:10.1385/0-89603-385-6:365

    PubMed  CAS  Google Scholar 

  • Ulmasov T, Hagen G, Guilfoyle TJ (1999) Activation and repression of transcription by auxin-response factors. PNAS 96(10):5844–5849

    Article  PubMed  CAS  Google Scholar 

  • Vlot AC, Dempsey DMA, Klessig DF (2009) Salicylic acid, a multifaceted hormone to combat disease. Annu Rev Phytopathol 47:177–206

    Article  PubMed  CAS  Google Scholar 

  • Wang D, Amornsiripanitch N, Dong X (2006) A genomic approach to identify regulatory nodes in the transcriptional network of systemic acquired resistance in plants. PLoS Pathog 2:123

    Article  Google Scholar 

  • Wang D, Pajerowska-Mukhtar K, Culler AH, Dong X (2007) Salicylic acid inhibits pathogen growth in plants through repression of the auxin signaling pathway. Curr Biol 17:1784–1790

    Article  PubMed  CAS  Google Scholar 

  • Wang SK, Bai YH, Shen CJ, Wu YR, Zhang SN, Jiang DA, Guilfoyle TJ, Chen M, Qi YH (2010) Auxin-related gene families in abiotic stress response in Sorghum bicolor. Funct Integr Genomic 10:533–546

    Article  CAS  Google Scholar 

  • Xuan N, Jin Y, Zhang H, Xie Y, Liu Y, Wang G (2011) A putative maize zinc-finger protein gene, ZmAN13, participates in abiotic stress response. Plant Cell Tiss Organ Cult 107:101–112

    Article  CAS  Google Scholar 

  • Yusuf M, Hasan SA, Ali B, Hayat S, Fariduddin Q, Ahmad A (2008) Effect of salicylic acid on salinity-induced changes in Brassica juncea. J Integr Plant Biol 50(9):1096–1102

    Article  PubMed  CAS  Google Scholar 

  • Zhang ZQ, Li Q, Li ZM, Staswick PE, Wang MY, Zhu Y, He ZH (2007) Dual regulation role of GH3.5 in salicylic acid and auxin signaling during Arabidopsis-Pseudomonas syringae interaction. Plant Physiol 145:450–464

    Article  PubMed  CAS  Google Scholar 

  • Zhao B, Liang R, Ge L, Li W, Xiao H, Lin H, Ruan K, Jin Y (2007) Identification of drought-induced microRNAs in rice. Biochem Biophys Res Co 354(2):585–590

    Article  CAS  Google Scholar 

  • Zhou J, Li FX, Chen S (2010) Cloning and expression analysis of the PVY resistance-related gene NtPsaN in Tobacoo (Nicotiana tabacum). Sci Agric Sin 43(16):3323–3330

    CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the Projects of National Natural Science Foundation of China (30972002, 31071798) and the Committee of Science and Technology of Chongqing (2011BA1024).

Author information

Authors and Affiliations

  1. Genetic Engineering Research Center, Bio-Engineering College, Key Laboratory of Functional Gene and New Regulation Technologies Under Chongqing Municipal Education Commission, Chongqing University, Chongqing, 400030, China

    Chunwen Yang, Wei Deng, Ning Tang, Xiaomin Wang, Fang Yan, Dongbo Lin & Zhengguo Li

Authors
  1. Chunwen Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wei Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ning Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiaomin Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Fang Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Dongbo Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Zhengguo Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhengguo Li.

Additional information

Chunwen Yang and Wei Deng contributed equally to this work and are considered co-first authors.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yang, C., Deng, W., Tang, N. et al. Overexpression of ZmAFB2, the maize homologue of AFB2 gene, enhances salt tolerance in transgenic tobacco. Plant Cell Tiss Organ Cult 112, 171–179 (2013). https://doi.org/10.1007/s11240-012-0219-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-012-0219-5

Keywords

Search

Navigation