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Shoot organogenesis and plant regeneration in Metabriggsia ovalifolia

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Abstract

An efficient propagation and regeneration system via direct shoot organogenesis for an endangered species, Metabriggsia ovalifolia, was established. High activity cytokinins [6-benzyladeneine (BA) and thidiazuron (TDZ)] and low activity auxins [α-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA)] could directly induce adventitious shoots from leaf or petiole explants within 5 weeks. Cytokinins (TDZ or BA) combined with auxin (NAA) in the induction media induced more adventitious shoots than when auxins or cytokinins were used alone. Adventitious shoots could be induced and also mass-propagated on media containing 2.5–5.0 μM TDZ (or BA) and 0.25–0.5 μM NAA. Adventitious roots differentiated at the proximal end of shoots on rooting media containing half-strength MS salts and 0.5 μM IBA, 0.5 μM NAA, 0.1% activated charcoal or no plant growth regulators. Over 90% of plantlets survived following acclimatization and transfer to a potting mixture (1:1, sand:vermiculite) in basins.

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Abbreviations

2, 4-D:

2, 4-Dichloro-phenoxyacetic acid

BA:

6-Benzyladeneine

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

KIN:

Kinetin

NAA:

α-Naphthaleneacetic acid

TDZ:

Thidiazuron

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Authors and Affiliations

  1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, China

    Guohua Ma, Jinfeng Lü, Xinhua Zhang & Jietang Zhao

  2. Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho, Ikenobe 2393, Kagawa-ken, 761-0795, Japan

    Jaime A. Teixeira da Silva

  3. Graduate University of Chinese Academy of Sciences, 100039, Beijing, China

    Jinfeng Lü

Authors
  1. Guohua Ma
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  2. Jaime A. Teixeira da Silva
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  3. Jinfeng Lü
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  4. Xinhua Zhang
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  5. Jietang Zhao
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Correspondence to Guohua Ma.

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Ma, G., Teixeira da Silva, J.A., Lü, J. et al. Shoot organogenesis and plant regeneration in Metabriggsia ovalifolia . Plant Cell Tiss Organ Cult 105, 355–361 (2011). https://doi.org/10.1007/s11240-010-9875-5

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  • DOI: https://doi.org/10.1007/s11240-010-9875-5

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