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Overexpression of a yeast cadmium factor 1 (YCF1) enhances heavy metal tolerance and accumulation in Brassica juncea

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Abstract

A yeast cadmium factor 1 (YCF1) is a member of the ATP-binding cassette (ABC) transporter family associated with multi-drug resistance, and it is localized at the vacuolar membrane in Saccharomyces cerevisiae. To determine ability to increase heavy metal tolerance and accumulation, YCF1 was introduced into Brassica juncea plants by Agrobacterium-mediated genetic transformation. YCF1 gene presence in transgenic plants was demonstrated by polymerase chain reaction (PCR). Reverse transcriptase-PCR analysis confirmed YCF1 gene expression in the transgenic plants, but the degree of YCF1 expression varied among the lines. YCF1 overexpression in B. juncea conferred enhanced tolerance to cadmium (Cd[II]) and lead (Pb[II]) stress. Transgenic B. juncea seedlings showed 1.3- to 1.6-fold tolerance to Cd stress and 1.2- to 1.4-fold tolerance to Pb stress compared to wild type (WT) plants (per gram fresh weight). Most importantly, the shoot tissues of transgenic seedlings contained about 1.5- to 2-fold higher Cd(II) and Pb(II) levels than those of WT, demonstrating significantly increased accumulation of both Cd(II) and Pb(II) in transgenic plants.

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Abbreviations

ABC:

ATP-binding cassette

BA:

6-Benzyladenine

HPT:

Hygromycin phosphotransferase

ICP-MS:

Inductively coupled plasma mass spectrophotometer

MS:

Murashige and Skoog medium

NAA:

α-Naphthaleneacetic acid

OD:

Optical density

RT–PCR:

Reverse transcriptase-polymerase chain reaction

YCF1:

Yeast cadmium factor 1

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Acknowledgments

This research was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2007-211). Special thanks to Dr. JH Lee and Dr. JW Ahn for their valuable suggestions during the experiments.

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Author notes

  1. Mohammed Shafi Ullah Bhuiyan

    Present address: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong-gu, Daejeon, 305-806, South Korea

Authors and Affiliations

  1. Department of Agricultural Biology, Chungnam National University, Yuseong-gu, Daejeon, 305-764, South Korea

    Mohammed Shafi Ullah Bhuiyan & Kwan Sam Choi

  2. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong-gu, Daejeon, 305-806, South Korea

    Sung Ran Min, Won Joong Jeong & Jang R. Liu

  3. Department of Bioscience and Biotechnology, Chungnam National University, Yuseong-gu, Daejeon, 305-764, South Korea

    Sayeda Sultana

  4. POSTECH-UZH Cooperative Laboratory, Department of Integrative Bioscience and Biotechnology, Pohang University of Science and Technology, Pohang, 790-784, South Korea

    Won Yong Song & Youngsook Lee

  5. Department of Horticulture, Chungnam National University, Yuseong-gu, Daejeon, 305-764, South Korea

    Yong Pyo Lim

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  1. Mohammed Shafi Ullah Bhuiyan
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  2. Sung Ran Min
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Corresponding author

Correspondence to Jang R. Liu.

Additional information

M. S. U. Bhuiyan and S. R. Min contributed equally to this work.

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Bhuiyan, M.S.U., Min, S.R., Jeong, W.J. et al. Overexpression of a yeast cadmium factor 1 (YCF1) enhances heavy metal tolerance and accumulation in Brassica juncea . Plant Cell Tiss Organ Cult 105, 85–91 (2011). https://doi.org/10.1007/s11240-010-9845-y

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  • DOI: https://doi.org/10.1007/s11240-010-9845-y

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