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High copper levels in the medium improves shoot bud differentiation and elongation from the cultured cotyledons of Capsicum annuum L.

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Abstract

The effect of copper sulphate on differentiation and elongation of shoot buds from cotyledonary explants of Capsicum annuum L. cv X-235 was investigated. Shoot buds were induced on medium supplemented with 22.2 μM BAP and 14.7 μM PAA. Elongation of shoot buds was obtained on MS medium containing 13.3 μM BAP + 0.58 μM GA3. Both shoot induction and elongation media were supplemented with different levels of CuSO4 (0–5 μM). The levels of CuSO4 in the induction as well as elongation medium highly influenced the shoot bud formation and their subsequent elongation. Highest number of shoot buds per explant was obtained when the concentration of CuSO4 was increased 30 times to the normal MS level. Shoot buds formation frequency i.e., the number of shoots formed per explant was increased two fold as compared to those formed on control. Elongation both in terms of percentage and length of shoots was better than that on control. Healthy elongated shoots were rooted on MS medium supplemented with 5.7 μM IAA. Rooted plantlets were transferred to field conditions.

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Abbreviations

BAP:

6-benzylaminopurine

MS:

Murashige and Skoog basal medium (1962)

PAA:

Phenyl acetic acid

GA3 :

Gibberellic acid

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Acknowledgement

Research fellowship to A. Joshi awarded by Council of Scientific and Industrial Research (CSIR), New Delhi is gratefully acknowledged.

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  1. Experimental Morphogenesis and Tissue Culture Laboratory, Department of Botany, University of Rajasthan, Jaipur, Rajasthan, 302 004, India

    A. Joshi & S. L. Kothari

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  1. A. Joshi
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  2. S. L. Kothari
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Correspondence to S. L. Kothari.

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Joshi, A., Kothari, S.L. High copper levels in the medium improves shoot bud differentiation and elongation from the cultured cotyledons of Capsicum annuum L.. Plant Cell Tiss Organ Cult 88, 127–133 (2007). https://doi.org/10.1007/s11240-006-9171-6

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  • DOI: https://doi.org/10.1007/s11240-006-9171-6

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