Skip to main content
SpringerLink
Log in

Influence of NaClO on nutrient medium sterilization and on pineapple (Ananas comosus cv Smooth cayenne) behavior

  • Original Paper
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

A new protocol for the preparation and sterilization of media with sodium hypochlorite was tested for in vitro culturing of pineapple shoots. Total active chlorine concentrations equal to or higher than 0.0003%, as well as other asepsis procedures, provided complete sterilization of the medium. At a total active chlorine concentration of 0.0003%, pineapple shoots more than doubled both their biomass and the number of new shoots.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

IAA:

Indoleacetic acid

BAP:

Benzilaminopurine

IBA:

Indolbutyric acid

References

  • Araujo MC, Teixeira SL (1998) [Sterilization of nutrient medium for plant tissue culture in microwave oven].␣Reunião de Programação de Pesquisas do CCTA—RESUMOS. Novembro de 1998. Centro de Ciências e Tecnologias Agropecuárias, UENF, Campos dos Goytacazes, RJ, Resumo 154 [In Port.]

  • Araujo MC, Teixeira SL (1999) [Sterilization of nutrient medium for plant tissue culture in microwave oven]. IV Encontro de Iniciação Científica da UENF. 03 a 05 de agosto de 1999. Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ. Resumo D-29, Pg. 81 [In Port.]

  • Ball E (1953) Hydrolysis of sucrose by autoclaving medium the neglected aspect in the technique of culture of plant tissues. Bull Torrey Bot Club 80:409–411

    Article  CAS  Google Scholar 

  • Bioactivos Chemists CBQ Centro (1997) Diaz MG, Conzalez BMM, Salazar YE, Castanedo CNR, Cueto SM of la C., González LCM, Machado RRM, Martin TEL, Ramirez DA: Microcide composition. AO1N43/00. ASS EP0920804. 23/06/1997. European Patent Office. http://v3.espacenet.com/texto? AB. Accessed in 10/05/2004

  • Burger DW (1988) Guidelines for autoclaving liquid medium used in plant tissue culture. HortScience 23: 1066–1068

    Google Scholar 

  • Ferguson JD, Street HE, David SB (1958) The carbohydrate nutrition of tomato roots. V. The promotion and inhibition of excised root growth by various sugars and sugar alcohols. Ann Bot 22:513–524

    CAS  Google Scholar 

  • Gonzales EJ (2005) [Biofactory, in Misiones, the first of the country]. http://www.pymesdigital.com.ar/opinion/opi58,.htm. Assessed in 20/06/2005 [In Span.]

  • Liau DF, Boll WG (1970) Callus and cell suspension culture of bush bean (Phaseolus vulgaris). Can J Bot 48:1119–1130

    Article  Google Scholar 

  • Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18:100–127

    Article  CAS  Google Scholar 

  • Murashige TM, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nissen SJ, Sutter EG (1988) Stability of IAA and IBA in nutrient medium after autoclaving and after storage under various environmental conditions. HortScience 23:758

    Google Scholar 

  • Plant Cell Technology Inc (1995) Assaf ZC, Kishor NP: Compositions and methods to prevent microbial contamination of plant tissue culture medium. C12N005/00; C12N005/02. US 5,750,402. 02/06/1995, 12/05/1998. United States Patent and Trademark Office—USPTO. http://www.uspto.gov/patft/index.html. Accessed in 10/05/2004

  • Salisbury FB, Ross C (1969) Plant physiology, Wadsworth Publishing co, Belmont

  • Singh M, Krikorian AD (1981) White´s standard nutrient solution. Ann Bot 47:133–139

    CAS  Google Scholar 

  • Snow R (1985) Improvements in methods for the germination of orchid seeds. Amer Orch Soc Bull 54:178–181

    Google Scholar 

  • Street HE, Lowe JS (1950) The carbohydrate nutrition of tomato roots. II The mechanism of sucrose absorption by excised roots. Ann Bot 14:307–329

    CAS  Google Scholar 

  • Teixeira SL (2005a) [Chemical sterilization of culture media]. Hort Bras (Suppl) 23:668 [In Port.]

  • Teixeira SL, Campanati M, Teixeira MT, Almeida RF (2005b) [Sterilization of nutrient medium for plant tissue culture, by combining chemical sterilants with microwave oven]. Rev Ceres 52:343–349 [In Port.]

    Google Scholar 

  • Teixeira SL, Teixeira MT, Ribeiro JM (2005c) Chemical sterilization of culture medium: 1.culture flasks and covers—rinsing with chlorinated water. Hort Bras (Suppl) 23:591

    Google Scholar 

  • Teixeira SL, Teixeira MT, Ribeiro JM (2005d) Chemical sterilization of culture medium: 2 addition of sodium hypochlorite to the medium. Hort Bras (Suppl) 23:591

    Google Scholar 

  • Teixeira SL, Teixeira MT,Ribeiro JM (2005e) Chemical sterilization of culture medium: 3 flasks filling and medium inoculation under non-sterile environment. Hort Bras (Suppl) 23:592

    Google Scholar 

  • Teixeira SL,Torres AC (1998) [Plant tissue culture laboratory organization]. In:Torres AC, Caldas LS & Buso JA (ed): Cultura de tecidos e transformação genética de plantas, Vol. 1 (pp 71–86). EMBRAPA, Brasília [In Port.]

  • Tisserat B, Jones D,Galletta PD (1992) Microwave sterilization of plant tissue culture medium. HortScience 27:358–361

    Google Scholar 

  • Weatherhead MA, Burdon J, Henshaw GG (1979) Effects of activated charcoal as an additive plant tissue culture media. Z Pflanzenphysiol 94:399–406

    CAS  Google Scholar 

  • White PR (1943) Nutrient deficiency studies and an improved inorganic nutrient medium for cultivation of excised tomato roots. Growth 7:53

    CAS  Google Scholar 

  • Yanagawa T, Nagai M, Ogino T, Maeguchi R (1995) Application of disinfectants to orchid seeds, plantlets and medium as a means to prevent in vitro contamination. Lyndleyana 10:33–36

    Google Scholar 

Download references

Acknowledgements

We are indebted to FENORTE/TECNORTE and FAPERJ (Brazil) for funding this research work.

Author information

Authors and Affiliations

  1. Center of Sciences and Agricultural Technologies, Universidade Estadual do Norte Fluminense, 28013-600, Campos dos Goytacazes, RJ, Brazil

    Silvio Lopes Teixeira, Juliana Martins Ribeiro & Márcia Torres Teixeira

Authors
  1. Silvio Lopes Teixeira
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Juliana Martins Ribeiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Márcia Torres Teixeira
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Silvio Lopes Teixeira.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Teixeira, S.L., Ribeiro, J.M. & Teixeira, M.T. Influence of NaClO on nutrient medium sterilization and on pineapple (Ananas comosus cv Smooth cayenne) behavior. Plant Cell Tiss Organ Cult 86, 375–378 (2006). https://doi.org/10.1007/s11240-006-9121-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-006-9121-3

Keywords

Search

Navigation