Abstract
The growth and gender in the gametophyte of Blechnum spicant L. were strongly affected by its origin, spore or mature homogenized gametophytes, and also by the addition of plant growth regulators to the culture medium. Spore-derived gametophytes cultured in Murashige and Skoog liquid medium were females and heart-shaped, whereas those derived from mature homogenized gametophytes were shorter and male or asexual (1:3) because of the release of antheridiogen to the culture medium. In the latter, maleness was especially increased by the addition of BA. This cytokinin is a strong inductor of maleness in homogenized cultures; however, even though BA influenced sexual organ formation in spore-derived gametophytes, it does not change the␣female sexual pattern that these gametophytes have. Separate male and female populations of gametophytes were obtained in this work.
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Abbreviations
- IAA:
-
Indol-3-acetic acid
- BA:
-
6-Benzylaminopurine
- GA3 :
-
Gibberellic acid
- GA4+7 :
-
Mixture of GA4 and GA7 gibberellins
- MS:
-
Murashige and Skoog mineral medium
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Acknowledgements
Our thanks to Dr Ana Elisa Valdés for her help with statistical analyses and also for her contribution in improving this manuscript. This research was supported by the National Scientific Program Ramon y Cajal.
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Menéndez, V., Revilla, M.A. & Fernández, H. Growth and gender in the gametophyte of Blechnum spicant L. Plant Cell Tiss Organ Cult 86, 47–53 (2006). https://doi.org/10.1007/s11240-006-9095-1
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DOI: https://doi.org/10.1007/s11240-006-9095-1