Abstract
The optimal conditions for fusion of leaf protoplasts of Populus alba, Betula platyphylla, and Alnus firma by electric treatment were alternate current (AC) 200 V cm−1 in 2.5 mM CaCl2 for a pearl chain formation and direct current (DC) pulse of 100 μs at 2 kV cm−1 After interfamilial cell fusion treatment, colonies were obtained using liquid media containing 2,4-D or NAA as an auxin and BA or CPPU as a cytokinin at 0.1, 1, or 10 µM in MS (Murashige and Skoog (1962) Physiol. Plant. 15: 473--497), 1/2salt MS, or NH4NO3-free MS containing 0.6 M mannitol and 3% sucrose (totaling 147 combinations). Two shoots after electric cell fusion treatment between P. alba and B. platyphylla, and 12 regenerated plants after electric cell fusion between P. alba and A. firma, were obtained from colonies induced on agar medium containing NAA, IBA, CPPU, and BA. Seven lines of the latter 12 plants which were regenerated later cultured in vitro had serrated leaves different from those of P. alba.
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Abbreviations
- BA:
-
benzyladenine
- CPPU:
-
N-(2-chloro-4-pyridyl)-N‘-phenylurea
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IBA:
-
indolebutyric acid
- NAA:
-
naphthaleneacetic acid
References
NJ Bate SB Rood TJ Blake (1988) ArticleTitleGibberellins and heterosis in poplar Can. J. Bot. 66 1148–1152
Ito K, Tatemichi Y& Shibata M (1986) Regeneration of poplar-like plants from fusion between Populus (hybrid) and Hibiscus. Abst. 6th Int. Congress of Plant Tissue & Cell Culture, 389
H Kisaka T Kameya (1998) ArticleTitleCold and salt tolerance of somatic hybrid calli between Barley (Hordeum vulgare L.) and carrot (Daucus carota L.) Breed. Sci. 48 11–15
H Kisaka M Kisaka A Kanno T Kameya (1998) ArticleTitleIntergeneric somatic hybridization of rice (Oryza sativa L.) and barley (Hordeum vulgare L.) by protoplast fusion Plant Cell Rep. 17 362–367 Occurrence Handle10.1007/s002990050407
H Kunitake T Nakashima K Mori M Tanaka A. Saito M Mii (1996) ArticleTitleProduction of interspecific somatic hybrid plants between Asparagus officinalis and A. macowanii through electrofusion Plant Sci. 116 213–222 Occurrence Handle10.1016/0168-9452(96)04389-0
T Moriguchi T Hidaka M Omura T Motomura T Akihama (1996) ArticleTitleGenotype and parental combination influence efficiency of cybrid induction in Citrus by electrofusion HortScience 31 275–278
T Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassay with tobacco tissue culture Physiol. Plant. 15 473–497
UK Nadjimov IM Scott GN Fatkhullaeva MS Mirakhmedov BU Nasirullaev D Musaev (1999) ArticleTitleConditioning of fasciation by gibberellin and genotype in cotton (Gossypium hirsutum L.) J. Plant Growth Regul. 18 45–48 Occurrence Handle10467019
R Pattanavibool K Klimaszewska P Aderkas Particlevon (1998) ArticleTitleInterspecies protoplast fusion in Larix: comparison of electric and chemical methods In Vitro Cell. Dev. Biol. Plant 34 212–217
H Sasamoto Y Hosoi K Ishii T Sato A Saito (1989a) ArticleTitleFactors affecting the formation of callus from leaf protoplasts of Populus alba J. Jpn. For. Soc. 71 449–455
Sasamoto H., Hosoi Y, Ishii K, Sato T & Saito A (1989b) Organ differentiation from the protoplasts-derived calli and from the suspension cultures of Populus alba leaves. Trans. 100th Metg. Jpn. For. Sci.: 529–530
Sasamoto H, Hosoi Y, Koshioka M (1995)Endogenous levels of four plant hormones may affect the culture conditions of poplar protoplasts to regenerate plants. In: Terzi M, Cella R, Falavigna A (eds) Current Issues in Plant Molecular and Cellular Biology. Kluwer Academic Publishers (pp 481–486)
H Sasamoto Y Wakita S Yokota N Yoshizawa (2000) ArticleTitleLarge electro-fused protoplasts of Populus alba selected by a micromanipulator: Techniques and some characteristics of cells and their regenerants J. For. Res. 5 265–270
H Sasamoto S Ogita Y Wakita M Fukui (2002) ArticleTitleEndogenous levels of abscisic acid and gibberellins in leaf protoplasts competent for plant regeneration in Betula platyphylla and Populus alba Plant Growth Regul. 38 195–201 Occurrence Handle10.1023/A:1021515630671
M Tamura R Tao A Sugiura (1998) ArticleTitleProduction of somatic hybrids between Diospyros glandulosa and D. kaki by protoplast fusion Plant Cell Tiss. Org. Cult. 54 85–91
Y Teng VR Timmer (1993) ArticleTitleGrowth and nutrition of hybrid poplar in response to phosphorus, zinc, and gibberellic acid treatments Forest Sci. 39 252–259
M Thie’baut (2000) ArticleTitleA foliar morphometric approach to the study of Salicaceae The Botanical Review 66 423–439
T Tzfira W Wang A Altman (2000) Genetic transformation of Populus toward improving plant performance and drought tolerance SM Jain SC Minocha (Eds) Molecular and Biology of Woody Plants NumberInSeries 2 Kluwer Academic Publishers UK 135–160
Y Wakita (1997) ArticleTitlePlant regeneration from protoplasts of broad-leaved trees Bull. of Utsunomiya Univ. Forests 33 55–108
Y Wakita H Sasamoto S Yokota N Yoshizawa (1996a) ArticleTitleCallus proliferation from protoplasts isolated from cell suspension cultures of Alnus firma Sieb Plant Tiss. Cult. Lett. 13 279–284
Y Wakita H Sasamoto S Yokota N Yoshizawa (1996b) ArticleTitlePlantlet regeneration from mesophyll protoplasts of Betula platyphylla var japonica Plant Cell Rep. 16 50–53
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Wakita, Y., Yokota, S., Yoshizawa, N. et al. Interfamilial cell fusion among leaf protoplasts of Populus alba, Betula platyphylla and Alnus firma: assessment of electric treatment and in vitro culture conditions. Plant Cell Tiss Organ Cult 83, 319–326 (2005). https://doi.org/10.1007/s11240-005-8428-9
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DOI: https://doi.org/10.1007/s11240-005-8428-9