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High content of endogenous cytokinins stimulates activity of enzymes and proteins involved in stress response in Nicotiana tabacum

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Abstract

Transgenic Pssu-ipt tobacco with elevated content of endogenous cytokinins grown under in vitro conditions exhibited elevated activities of antioxidant enzymes (i.e. catalase, ascorbate peroxidase, guaiacol and syringaldazine peroxidase, glutathione reductase) and some of enzymes involved in anaplerotic pathways such as glucose-6-phosphate dehydrogenase, glycolate oxidase, NADP-malic enzyme, NADP-isocitrate dehydrogenase, and glutamate dehydrogenase compared to control non-transgenic SR1 tobacco. Higher activities of peroxidases, NADP-malic enzyme, and glutamate dehydrogenase were maintained in transgenic grafts after several weeks of the growth under ex vitro conditions, while transgenic rooted plants showed only the increase in activity of glycolate oxidase compared to control non-transformed tobacco. Total activities of superoxide dismutase were lower in both types of Pssu-ipt tobacco contrary to controls under both growth conditions. The presence of PR-1 protein and proteins with elevated activities of chitinase was proved in the extracellular fluid in both transgenic types under both in vitro and ex vitro conditions.

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Authors and Affiliations

  1. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Karlovce 1a, CZ-160 00 Praha 6, Czech Republic

    Helena Synková, Šárka Semorádová & Lenka Burketová

  2. Faculty of Sciences, Department of Plant Anatomy and Physiology, Charles University, Viničná 5, CZ-128 44 Praha 2, Czech Republic

    Šárka Semorádová

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  1. Helena Synková
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  2. Šárka Semorádová
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  3. Lenka Burketová
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Correspondence to Helena Synková.

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Synková, H., Semorádová, Š. & Burketová, L. High content of endogenous cytokinins stimulates activity of enzymes and proteins involved in stress response in Nicotiana tabacum . Plant Cell, Tissue and Organ Culture 79, 169–179 (2004). https://doi.org/10.1007/s11240-004-0657-9

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