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Hemostatic potential of latex proteases from Tabernaemontana divaricata (L.) R. Br. ex. Roem. and Schult. and Artocarpus altilis (Parkinson ex. F.A. Zorn) Forsberg

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Abstract

Pharmacological properties exhibited by latex of plants are due to various biologically active compounds including several proteolytic enzymes. Present study evaluates hemostatic potential of Tabernaemontana divaricata and Artocarpus altilis from Apocynaceae and Moraceae families respectively. The latex of these plants were initially subjected to dialysis and crude extracts were estimated for proteolytic activity using casein as the substrate. Mean caseinolytic activity for 100 μg of latex protein was found to be 56.16 ± 0.57 and 45 ± 0.3 U/h for T. divaricata and A. altilis respectively. Caseinolytic activity by both the plant extracts was higher than standard proteases, papain and trypsin. However the difference was significant (p < 0.05) with papain alone. Crude enzymes (CE) from both plants exhibited coagulant activity on human platelet poor plasma by recalcification time. A significant reduction in clotting time was exhibited by T. divaricata compared to A. altilis (p < 0.05). These results were further substantiated with fibrinogen agarose plate assay. Crude enzyme of both plants also hydrolyzed blood clot. Mean % of thrombolysis by T. divaricata was 80.75 ± 1.2 and that of A. altilis was 70.24 ± 1.52. Inhibition studies confirmed cysteine protease nature of CE. Comparative analysis revealed T. divaricata to be the best among the two for its hemostatic potential. This study scientifically validates the use of latex from these plants in the management of fresh cuts or wounds.

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Acknowledgments

The authors thank Prof. Leela Iyengar (Adjunct Professor, Jain University) for her valuable suggestions and fruitful discussions during the study. We are equally thankful to Dr. Krishna Venkatesh (Centre for Emerging Technologies, Jain University), for his help and support during the study. Financial assistance to Maheshwari Kumari Singh in the form of DST INSPIRE Fellowship, Government of India is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Biochemistry, Center for Post Graduate Studies, Jain University, 9th Main, 18th Cross, Jayanagar 3rd Block, Bangalore, Karnataka, India

    Maheshwari Kumari Singh, R. Usha, K. R. Hithayshree & O. S. Bindhu

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  1. Maheshwari Kumari Singh
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  2. R. Usha
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  3. K. R. Hithayshree
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  4. O. S. Bindhu
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Correspondence to O. S. Bindhu.

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Singh, M.K., Usha, R., Hithayshree, K.R. et al. Hemostatic potential of latex proteases from Tabernaemontana divaricata (L.) R. Br. ex. Roem. and Schult. and Artocarpus altilis (Parkinson ex. F.A. Zorn) Forsberg. J Thromb Thrombolysis 39, 43–49 (2015). https://doi.org/10.1007/s11239-013-1012-y

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