Abstract
Using a direct amplification of genomic DNA from two Brassica rapa forms, we obtained two homologs of the CONSTANS gene, which controls the photoperiodic induction of flowering in Arabidopsis plants. The cloned fragments of B. rapa genome were identified as members of the CONSTANS-LIKE1 class. By aligning the nucleotide sequences of the CONSTANS gene and its homologs, three classes, CONSTANS, CONSTANS-LIKE1, and CONSTANS-LIKE2, were distinctly discerned by their primary structure. The pattern of restriction fragment length polymorphisms (RFLP) of the CONSTANS homologs in B. carinata, B. juncea, B. napus, B. nigra, B. oleracea, and B. rapa were genome-specific; in addition, the CONSTANS homologs were classified by plant geographic origin, and we assume that such classification is related to plant photoperiodic response.
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Abbreviations
- CO:
-
CONSTANS
- COL:
-
CONSTANS-LIKE
- PCR:
-
polymerase chain reaction
- RFLP:
-
restriction fragment length polymorphism
REFERENCES
Van Nocker, S., The Molecular Biology of Flowering, Hort. Rev., 2001, vol. 27, pp. 1–39.
Simpson, G.G. and Dean, C., Arabidopsis, the Rosetta Stone of Flowering Time? Science, 2002, vol. 296, pp. 285–289.
Yanovsky, M.J. and Kay, S.A., Living by the Calendar: How Plants Know When to Flower, Nature Rev. Mol. Cell Biol., 2003, vol. 4, pp. 265–275.
Hayama, R. and Coupland, G., The Molecular Basis of Diversity in the Photoperiodic Flowering Responses of Arabidopsis and Rice, Plant Physiol., 2004, vol. 135, pp. 677–684.
Robson, F., Costa, M.M.R., Hepworth, S.R., Vizir, I., Pineiro, P., Reeves, P.H., Putterill, J., and Coupland, G., Functional Importance of Conserved Domains in the Flowering-Time Gene CONSTANS Demonstrated by Analysis of Mutant Alleles and Transgenic Plants, Plant J., 2001, vol. 28, pp. 619–631.
Hepworth, S., Valverde, F., Ravenscroft, D., Mouradov, A., and Coupland, G., Antagonistic Regulation of Flowering-Time Gene SOC1 by CONSTANS and FLC via Separate Promoter Motifs, EMBO J., 2002, vol. 21, pp. 4327–4337.
An, H., Roussot, C., Suarez-Lopez, P., Corbesier, L., Vincent, C., Pineiro, M., Hepworth, S. Mouradov, A., Justin, S., Turnbull, C., and Coupland, G. CONSTANS Acts in the Phloem to Regulate a Systemic Signal That Induces Photoperiodic Flowering of Arabidopsis, Development, 2004, vol. 131, pp. 3615–3626.
Ayre, B.G. and Turgeon, R., Graft Transmission of a Floral Stimulant Derived from CONSTANS, Plant Physiol., 2004, vol. 135, pp. 2271–2278.
Chailakhyan, M.Kh., Regulyatsiya tsveteniya vysshikh rastenii (Control of the Higher Plant Flowering), Moscow: Nauka, 1988.
Lang, A., Physiology of Flower Initiation, Encyclopedia of Plant Physiology, vol. 15A, Ruhland, W., Ed., Berlin: Springer-Verlag, 1965, pp. 1379–1536.
Lagercrantz, U. and Axelsson, T., Rapid Evolution of the Family of CONSTANS LIKE Genes in Plants, Mol. Biol. Evol., 2000, vol. 17, pp. 1499–1507.
Robert, L.S., Robson, F., Sharpe, A., Lydiate, D., and Coupland, G., Conserved Structure and Function of the Arabidopsis Flowering Time Gene CONSTANS in Brassica napus, Plant Mol. Biol., 1998, vol. 37, pp. 763–772.
Oesterberg, M.K., Shavorskaya, O., Lascoux, M., and Lagercrantz, U., Naturally Occurring Indel Variation in the Brassica nigra COL1 Gene Is Associated with Variation in Flowering Time, Genetics, 2002, vol. 161, pp. 299–306.
U., N., Genomic Analysis in Brassica with Special Reference to the Experimental Formation of B. napus and Peculiar Mode of Fertilization, Jpn. J. Bot., 1935, vol. 7, pp. 389–452.
Martynov, V.V. and Khavkin, E.E., Two Homologs of the FLOWERING LOCUS C Gene from Leaf Mustard (Brassisa juncea Thell.), Fiziol. Rast. (Moscow), 2004, vol. 51, pp. 262–268 (Russ. J. Plant Physiol., Engl. Transl.).
Nei, M. and Li, W.H., Mathematical Model for Studying Genetic Variation in Terms of Restriction Endonucleases, Proc. Natl. Acad. Sci. USA, 1979, vol. 76, pp. 5269–5273.
Van de Peer, Y. and de Wachter, R., TREECON for Windows: A Software Package for the Construction and Drawing of Evolutionary Trees for the Microsoft Windows Environment, Comp. Appl. BioSci., 1994, vol. 10, pp. 569–570.
Lagercrantz, U., Oesterberg, M.K., and Lascoux, M., Sequence Variation and Haplotype Structure at the Putative Flowering-Time Locus COL1 of Brassica nigra, Mol. Biol. Evol., 2002, vol. 19, pp. 1474–1482.
Westman, A. and Kresovich, S., Simple Sequence Repeat (SSR)-Based Marker Variation in Brassica nigra Genebank Accessions and Weed Populations, Euphytica, 1999, vol. 109, pp. 85–92.
Axelsson, T., Shavorskaya, O., and Lagercrantz, U., Multiple Flowering Time QTLs within Several Brassica Species Could Be the Result of Duplicated Copies of One Ancestral Gene, Genome, 2001, vol. 44, pp. 856–864.
Schranz, M.E. and Osborn, T.C., De Novo Variation in Life-History Traits and Responses to Growth Conditions of Resynthesized Polyploid Brassica napus (Brassicaceae), Am. J. Bot., 2004, vol. 91, pp. 174–183.
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Translated from Fiziologiya Rastenii, Vol. 52, No. 2, 2005, pp. 274–281.
Original Russian Text Copyright © 2005 by Martynov, Khavkin.
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Martynov, V.V., Khavkin, E.E. Polymorphism of the CONSTANS gene in Brassica plants. Russ J Plant Physiol 52, 242–248 (2005). https://doi.org/10.1007/s11183-005-0037-2
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DOI: https://doi.org/10.1007/s11183-005-0037-2