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The effect of cadmium on CO2 exchange, variable fluorescence of chlorophyll, and the level of antioxidant enzymes in pea leaves

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Abstract

CO2 exchange, variable chlorophyll fluorescence, the intensity of lipid peroxidation (POL), and the activity of antioxidant enzymes in leaves of two-week-old pea seedlings (Pisum sativum L.) exposed to 0.01, 0.1, and 1 mM aqueous solutions of Cd(NO3)2 for 2 h were studied. Incubation with Cd2+ ions resulted in a reduction of the maximum quantum efficiency of photosynthesis, CO2 uptake rate, and photosystem II (PSII) activity, as assessed by F v/F 0 ratio. The intensity of POL in leaves of all treated seedlings was below or close to the control level. The activity of superoxide dismutase (SOD) and glutathione reductase (GR) increased in all treatments; the activity of ascorbate peroxidase (AP) exceeded the control level only in seedlings exposed to the high Cd2+ concentration (1 mM), and the activity of peroxidase increased at the low concentration (0.01 mM). We found that the reduction in the activity of the photosynthetic apparatus under conditions of 2-h-long Cd2+-induced stress was not related to an intensification of POL processes. It was concluded that stimulation of the activity of antioxidant enzymes—SOD, GR, AP, and peroxidase—is a pathway for pea plant adaptation to toxic effect of cadmium.

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Abbreviations

AP:

ascorbate peroxidase

GR:

glutathione reductase

POL:

peroxidation of lipids

PSII:

photosystem II

ROS:

reactive oxygen species

SOD:

superoxide dismutase

TBA:

thiobarbituric acid

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Authors and Affiliations

  1. Institute of Basic Problems of Biology, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    T. I. Balakhnina, A. A. Kosobryukhov, A. A. Ivanov & V. D. Kreslavskii

  2. All-Russian Institute of Breeding and Seed Growing of Vegetable Cultures, Russian Academy of Agricultural Sciences, Lesnoi gorodok, Moscow oblast, Russia

    T. I. Balakhnina

Authors
  1. T. I. Balakhnina
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  2. A. A. Kosobryukhov
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  3. A. A. Ivanov
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  4. V. D. Kreslavskii
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Translated from Fiziologiya Rastenii, Vol. 52, No. 1, 2005, pp. 21–26.

Original Russian Text Copyright © 2005 by Balakhnina, Kosobryukhov, Ivanov, Kreslavskii.

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Balakhnina, T.I., Kosobryukhov, A.A., Ivanov, A.A. et al. The effect of cadmium on CO2 exchange, variable fluorescence of chlorophyll, and the level of antioxidant enzymes in pea leaves. Russ J Plant Physiol 52, 15–20 (2005). https://doi.org/10.1007/s11183-005-0003-z

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  • DOI: https://doi.org/10.1007/s11183-005-0003-z

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