Specific features of sorption of bovine serum albumin on hydroxyapatite doped with zinc ions, and the possibilities of its use as an intermediate binding protein for heterocyclic ligands

Abstract

In order to characterize hydroxyapatite (HAP) as a possible carrier of medicinal drugs and radionuclides, the possibility of using HAP modified with zinc ions (HAP-Zn) for the sorption of albumin, in particular, bovine serum albumin (BSA), was considered, using the latter as an intermediate binding protein in complex with a heterocyclic ligand of medical purpose. The kinetics of sorption of albumin correspond to the pseudo-second order, and the parameters of the sorption isotherm can be described using calculations based on the Langmuir theory of monolayer adsorption. The results of experiments on the sequential and joint sorption of a heterocyclic ligand on HAP-Zn in the presence of BSA are ambiguous about the sorption binding of this ligand to the sorbent, however, an interaction (binding) between the starting BSA and the ligand was present.