Abstract
All the equilibrium conformations of 34 analogues of acetylcholine (ACh) with the general formula R-C(O)O-Alk-N+(CH3)3 are calculated by the method of molecular mechanics. In the series R-C(O)O-(CH2)2-N+(CH3)3, a reliable correlation is found between the molecular volume of the substrate and the rate of its hydrolysis by acetylcholinesterase (AChE); the absence of such a correlation is demonstrated for butyryl-cholinesterase (BChE). Theoretical conformational analysis confirms that the completely extended tt conformation of ACh is productive for the hydrolysis by AChE, which agrees with the results of X-ray analysis of AChE. AChE is shown to hydrolyze only those substrates that form equilibrium conformers compatible in the mutual arrangement of trimethylammonium group, carbonyl carbon, and carbonyl oxygen with the tt conformation of ACh; in this case, the rate of substrate hydrolysis depends on the total population of these conformers. A reliable correlation was found between the population of the semifolded (tg −) conformation of the choline moiety of substrate molecules and rate of their BChE hydrolysis. In a series of CH3-C(O)O-Alk-N+(CH3)3, the rate of BChE hydrolysis is demonstrated to depend on the total population of conformations compatible in the mutual arrangement of functionally important atoms with the tg − conformation of ACh. The tg − conformation of ACh is concluded to be productive for BChE hydrolysis. Similar orientations of the substrate molecules relative to the catalytic triads of both AChE and BChE are proven to coincide upon the substrate productive sorption in their active sites. It is hypothesized that the sorption stage is rate-limiting in cholinesterase hydrolysis and the enzyme hydrolyzes the ACh molecule in its energetically favorable conformation.
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Abbreviations
- ACh:
-
acetylcholine
- AChE:
-
acetylcholnesterase
- BChE:
-
butyrylcholinesterase
- TCA:
-
theoretical conformational analysis
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Translated from Bioorganicheskaya Khimiya, Vol. 31, No. 5, 2005, pp. 466–473.
Original Russian Text Copyright © 2005 by Belinskaya, Shestakova.
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Belinskaya, D.A., Shestakova, N.N. Theoretical Conformational Analysis in the Determination of Productive Conformations of Substrates for Acetylcholinesterase and Butyrylcholinesterase. Russ J Bioorg Chem 31, 419–425 (2005). https://doi.org/10.1007/s11171-005-0058-9
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DOI: https://doi.org/10.1007/s11171-005-0058-9