Abstract
N-(9-Fluorenylmethoxycarbonyl)-ω-aminoalkyl-, N-(9-fluorenylmethoxycarbonyl)-8-amino-3,6-dioxaoctyl, and N-[(9-fluorenylmethoxycarbonyl)-6-aminohexanoyl]-2-aminoethyl triphosphates were synthesized. All of them were shown to be the substrates of the calf thymus terminal deoxynucleotidyl transferase. Their substrate properties depend on the length and structure of the linker between the 9-fluorenylmethoxycarbonyl and triphosphate moieties.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- CDI:
-
1,1′-carbonyldiimidazole
- Fmoc:
-
9-fluorenylmethoxycarbonyl
- TDT:
-
terminal deoxynucleotidyl transferase
REFERENCES
Baltimore, D., Nature, 1974, vol. 248, pp. 409–411.
Arzumanov, A.A., Victorova, L.S., and Jasko, M.V., Nucleosides, Nucleotides & Nucleic Acids, 2000, vol. 19, pp. 1787–1793.
Arzumanov, A.A., Victorova, L.S., Jasko, M.V., Yesipov, D.S., and Krayevsky, A.A., Nucleic Acids Res., 2000, vol. 28, pp. 1276–1281.
Khandazhinskaya, A.L., Jasko, M.V., Shirokova, E.A., and Kukhanova, M.K., Collection Symposium Series, 2002, vol. 5, pp. 344–347.
Ludwig, I., Acta Biochim. Biophys. Acad. Sci. Hung., 1981, vol. 16, pp. 131–133.
Author information
Authors and Affiliations
Corresponding author
Additional information
__________
Translated from Bioorganicheskaya Khimiya, Vol. 31, No. 4, 2005, pp. 394–398.
Original Russian Text Copyright © 2005 by Khandazhinskaya, Kukhanova, Jasko.
Rights and permissions
About this article
Cite this article
Khandazhinskaya, A.L., Kukhanova, M.K. & Jasko, M.V. New Nonnucleoside Substrates for Terminal Deoxynucleotidyl Transferase: Synthesis and Dependence of Substrate Properties on Structure. Russ J Bioorg Chem 31, 352–356 (2005). https://doi.org/10.1007/s11171-005-0048-y
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/s11171-005-0048-y