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Primary Structures of Actinoporins from Sea Anemone Oulactis orientalis

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Abstract

Partial amino acid sequences of the actinoporins Or-A (136 aa) and Or-G (144 aa) isolated from the Sea of Japan sea anemone Oulactis orientalis were determined by sequencing the clones obtained by the amplification of genomic DNA and cDNA with primers specific to the N-terminal regions of the O. orientalis actinoporin sequences and to the C-terminal region, which is highly conservative in all the known actinoporin sequences. The complete structures of Or-A (165 aa) and Or-G (173 aa) were elucidated by sequencing the cDNA clones obtained by the rapid amplification of 3′-ends of cDNA. A comparative analysis of the amino acid sequences of the Oulactis actinoporins with those of actinoporins from tropical species revealed considerable differences in the structures of their N-terminal fragments and their membrane-binding sites. We believe that these differences could explain the lower hemolytic activities of Or-A and Or-G compared to those of actinoporins from the tropical species.

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Abbreviations

IPTG:

isopropyl β-D-thiogalactopyranoside

X-Gal:

5-bromo-4-chloro-3-indolyl β-D-galactopyranoside

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Authors and Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, pr. 100-letiya Vladivostoka 159, Vladivostok, 690022, Russia

    A. P. Il’ina, M. M. Monastyrnaya, M. P. Isaeva, K. V. Guzev, V. A. Rasskazov & E. P. Kozlovskaya

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  1. A. P. Il’ina
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  2. M. M. Monastyrnaya
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  3. M. P. Isaeva
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  4. K. V. Guzev
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  5. V. A. Rasskazov
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  6. E. P. Kozlovskaya
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Correspondence to A. P. Il’ina.

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__________

Translated from Bioorganicheskaya Khimiya, Vol. 31, No. 4, 2005, pp. 357–362.

Original Russian Text Copyright © 2005 by Il’ina, Monastyrnaya, Isaeva, Guzev, Rasskazov, Kozlovskaya

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Il’ina, A.P., Monastyrnaya, M.M., Isaeva, M.P. et al. Primary Structures of Actinoporins from Sea Anemone Oulactis orientalis . Russ J Bioorg Chem 31, 320–324 (2005). https://doi.org/10.1007/s11171-005-0044-2

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  • DOI: https://doi.org/10.1007/s11171-005-0044-2

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