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A fast and efficient stabilization of firefly luciferase on MIL-53(Al) via surface adsorption mechanism

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Abstract

Stabilization and immobilization on a proper support is essential in processing and production of enzymes. Luciferase is an unstable, thermally sensitive, and light-emitting enzyme that requires stabilization for efficiency and productivity in bio-luminescence reactions. In this work, a known and well-porous framework, MIL-53(Al), was used, for the first time, to support the immobilization of firefly luciferase. Interestingly, all the stability values were improved through the immobilization process, and the content of the loading enzyme on MIL-53(Al) was found to be significant. The results obtained indicated that the stabilization of firefly luciferase on MIL-53(Al) proceeded through the adsorption method. MIL-53(Al) was also characterized by the FT-IR, X-ray diffraction, FE-SEM, TEM, BET, and TGA techniques in order to determine the carboxylic groups and symmetric/asymmetric CO2, percentage of crystallinity and its purity, morphology, size, and shape of the MIL-53(Al) particles, and surface-to-area ratio and thermal gravimetry of support.

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Acknowledgements

The authors appreciate the support of Shahrood University of Technology and the Research Council of Tarbiat Modares University of Biological Sciences for the financial support of this work.

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Authors and Affiliations

  1. Faculty of Chemistry, Shahrood University of Technology, Shahrood, Semnan, Iran

    Zohreh Nowroozi-Nejad & Bahram Bahramian

  2. Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

    Saman Hosseinkhani

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  1. Zohreh Nowroozi-Nejad
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  2. Bahram Bahramian
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  3. Saman Hosseinkhani
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Correspondence to Bahram Bahramian.

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Nowroozi-Nejad, Z., Bahramian, B. & Hosseinkhani, S. A fast and efficient stabilization of firefly luciferase on MIL-53(Al) via surface adsorption mechanism. Res Chem Intermed 45, 2489–2501 (2019). https://doi.org/10.1007/s11164-019-03748-w

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